Bethyl Laboratories

KLC1 Polyclonal Antibody

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KLC1 Antibody (A302-711A) in WB

Detection of human KLC1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KLC1 antibody A302-711A used for WB at 0.4 µg/ml (A and B) and used for IP at 3 µg/mg lysate. KLC1 was also immunoprecipitated by rabbit anti-KLC1 antibody A302-710A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

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产品信息

A302-711A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

免疫沉淀 (IP)

2-5 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 523 and 573 of human kinesin light chain 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

tris citrate/phosphate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。