Invitrogen

KLF10 Monoclonal Antibody (3E11)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

5个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

KLF10 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

KLF10 Antibody (MA5-20125) in ICC/IF

Immunofluorescence analysis of HeLa cells using an anti-KLF10 monoclonal antibody (Product # MA5-20125). (antibody concentration 10 µg/mL).

Product Image Gallery

KLF10 Antibody — Figure 6KDM6A and KLF10 can build a positive feedback loop that is critically involved in podocyte dysfunction AQuantitative RT-PCR evaluation of KLF10 mRNA expression in kidney samples of wild-type and KLF10-knockout (KO) mice. **P < 0.0001, significant difference versus wild-type controls (Wilcoxon two-sample test; n = 8). BWestern blot analysis of KLF10 expression in kidney samples of wild-type or KLF10-KO mice treated with or without STZ at 8 weeks after the onset of diabetes. Presented experiments were performed at least three times independently. C Changes in body weight and blood glucose levels in wild-type or KLF10-KO mice with or without STZ treatment. No significant differences in body weights or blood glucose levels between diabetic wild-type mice and diabetic KLF10-KO mice were detected (Wilcoxon two-sample test; n = 8). D Levels of urinary protein excretion, weights of kidney, and levels of HbA1c in wild-type or KLF10-KO mice with or without STZ treatment. The mean relative kidney weight (%) shown in the study is determined as the percent of kidneys out of total body weight, and the HbA1c level is defined as the ratio of HbA1c to the total hemoglobin (% HbA1c; DCCT unit). *P < 0.05 versus untreated wild-type controls, # P < 0.05 versus STZ-treated wild-type mice (parametric ANOVA and a Bonferroni post hoc test; n = 8). E Immunofluorescence images of kidney sections stained with KLF10 and nephrin in wild-type or KLF10-KO mice untreated or treated with STZ. Scale b {KO}

产品信息

MA5-20125

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

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免疫细胞化学 (ICC/IF)

10 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

3E11

抗原

KLF10 (AAH11538.1, 111 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2607968

产品详细信息

Peptide Sequence: LSDTAKPHIA APFKEEEKSP VSAPKLPKAQ ATSVIRHTAD AQLCNHQTCP MKAASILNYQ NNSFRRRTHL NVEAARKNIP CAAVSPNRSK CERNTVADVD

靶标信息

KLF10 is a nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family with three C2H2-type zinc fingers. KLF10 is a transcriptional repressor involved in the regulation of cell growth and inhibits cell growth. It binds to the consensus sequence 5'-GGTGTG-3' and is induced by TGF-beta thus playing a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth. KLF10 is involved in inducing and promoting apoptosis through the mitochondrial apoptotic pathway and may inhibit Smad7 expression in bronchial epithelial cells. Ubiquitous expression of KLF10 is seen in most of the tissues. Reports suggest that it may mediate up-regulation of TGFBI in VHL-deficient tumors and other cancers. Over expression of KLF10 may be associated with renal clear cell carcinoma and other tumors. KLF11 is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction.

EMBO molecular medicine

Lin CL,Hsu YC,Huang YT,Shih YH,Wang CJ,Chiang WC,Chang PJ

Published figure using KLF10 monoclonal antibody (Product # MA5-20125) in Western Blot

Wed May 01 00:00:00 EDT 2019

生物信息学

蛋白别名: early growth response-alpha; EGR-alpha; Krueppel-like factor 10; Kruppel-like factor 10; TGFB-inducible early growth response protein 1; Transforming growth factor-beta-inducible early growth response protein 1; zinc finger transcription factor TIEG

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基因别名: EGR-alpha; EGRA; KLF10; TIEG; TIEG1

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UniProt ID: (Human) Q13118

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Entrez Gene ID: (Human) 7071

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功能

core promoter sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding protein binding metal ion binding C2H2 zinc finger transcription factor

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