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KLF4 Monoclonal Antibody (X1)

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抗体检测数据 (1)

KLF4 Antibody in Western Blot (WB)

KLF4 Antibody in Western Blot (WB)

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KLF4 Antibody (H00009314-M02) in WB

Western Blot analysis of KLF4 expression in transfected 293T cell line by KLF4 monoclonal antibody (M02), clone X1. Lane 1: KLF4 transfected lysate(50.1 KDa). Lane 2: Non-transfected lysate. .

KLF4 Antibody in Western Blot (WB)

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KLF4 Antibody in Western Blot (WB) — Western Blot analysis of KLF4 expression in transfected 293T cell line by KLF4 monoclonal antibody (M02), clone X1. Lane 1: KLF4 transfected lysate(50.1 KDa). Lane 2: Non-transfected lysate. .

产品信息

H00009314-M02

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

-

免疫细胞化学 (ICC/IF)

10-100 µg/mL

-

酶联免疫吸附实验 (ELISA)

10 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

X1

抗原

KLF4 (AAH29923, 221 a.a. approximately 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

产品详细信息

Product may be used with Western Blot (Transfected lysate).

Immunogen sequence: VLKASLSAPG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAVSSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD

靶标信息

Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: endothelial Kruppel-like zinc finger protein; Epithelial zinc finger protein EZF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (gut); RP11-150J11.1

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基因别名: EZF; GKLF; KLF4

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UniProt ID: (Human) O43474

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Entrez Gene ID: (Human) 9314

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core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription factor recruiting core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription cofactor binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding zinc ion binding phosphatidylinositol 3-kinase regulator activity histone deacetylase binding transcription regulatory region DNA binding C2H2 zinc finger transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter mesodermal cell fate determination negative regulation of cell proliferation epidermal cell differentiation positive regulation of gene expression negative regulation of gene expression negative regulation of phosphatidylinositol 3-kinase signaling negative regulation of muscle hyperplasia stem cell population maintenance post-embryonic camera-type eye development negative regulation of NF-kappaB transcription factor activity positive regulation of cellular protein metabolic process negative regulation of heterotypic cell-cell adhesion somatic stem cell population maintenance post-embryonic hemopoiesis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process fat cell differentiation regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hemoglobin biosynthetic process negative regulation of smooth muscle cell proliferation regulation of axon regeneration epidermis morphogenesis negative regulation of inflammatory response positive regulation of protein metabolic process negative regulation of protein kinase B signaling positive regulation of telomerase activity canonical Wnt signaling pathway negative regulation of response to cytokine stimulus cellular response to hydrogen peroxide negative regulation of ERK1 and ERK2 cascade cellular response to retinoic acid cellular response to growth factor stimulus cellular response to cycloheximide cellular response to laminar fluid shear stress negative regulation of cell migration involved in sprouting angiogenesis cellular response to peptide negative regulation of leukocyte adhesion to arterial endothelial cell negative regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of transcription regulatory region DNA binding