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LAMP2 Antibody (MA5-17861) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1) and NIH/3T3 (Lane 2). The blots were probed with Anti-LAMP2 Monoclonal Antibody (Product # MA5-17861, 1:1000 dilution) and detected by chemiluminescence using Sheep anti-Rat IgG Secondary Antibody, HRP conjugate (Product # PA1-28642, 0.2 µg/mL, 1:5000 dilution). A 110 kDa band corresponding to glycosylated form of LAMP2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 ... View More
产品信息
MA5-17861
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
M3/84
抗原
Immunoadsorbent purified mouse macrophage glycoprotein fraction.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539245
靶标信息
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon’s disease).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LGP-B; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; Lysosome-associated membrane glycoprotein 2; RP23-193O17.2
基因别名: CD107b; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-B; Mac3
UniProt ID: (Mouse) P17047
Entrez Gene ID: (Mouse) 16784
