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Invitrogen
LAT Monoclonal Antibody (LAT.10-17 (10-17)), eBioscience™
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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LAT Antibody (14-9967-82) in WB
Western blot was performed using Anti-LAT Monoclonal Antibody (LAT.10-17 (10-17)), eBioscience™ (Product # 14-9967-82) and 34kDa, 28kDa, 27kDa and 24kDa bands corresponding to LAT was observed in T cell line, Jurkat, and Natural Killer cells, NK-92, but not in B-cells (Raji and Daudi). Whole Cell Extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), Daudi (Lane 3) and NK-92 (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane ... View More
产品信息
14-9967-82
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
LAT.10-17 (10-17)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1235011
产品详细信息
Description: This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLCgamma, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.
Applications Reported: This LAT.10-17 (10-17) antibody has been reported for use in immunoblotting (WB) and immunoprecipitation (IP).
Applications Tested: LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36
基因别名: LAT; LAT1; pp36
UniProt ID: (Human) O43561
Entrez Gene ID: (Human) 27040
MAPK cascade
adaptive immune response
lymphocyte homeostasis
inflammatory response
immune response
integrin-mediated signaling pathway
Ras protein signal transduction
positive regulation of signal transduction
gene expression
calcium-mediated signaling
platelet activation
intracellular signal transduction
Fc-epsilon receptor signaling pathway
T cell activation
mast cell degranulation
positive regulation of GTPase activity
positive regulation of protein kinase activity
T cell receptor signaling pathway
regulation of T cell activation
