Invitrogen

LEF1 Recombinant Rabbit Monoclonal Antibody (13B7)

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LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LEF1 Antibody (MA5-50170) in IHC (P)

Immunohistochemistry analysis with a LEF1 monoclonal antibody (Product # MA5-50170) using a dilution of 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubat... View More

LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis with a LEF1 monoclonal antibody (Product # MA5-50170) using a dilution of 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.

LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis with a LEF1 monoclonal antibody (Product # MA5-50170) using a dilution of 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.

产品信息

MA5-50170

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

13B7

抗原

A synthesized peptide derived from Human LEF1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.33 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_3092733

靶标信息

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DKFZp586H0919; FLJ46390; HGNC:6551; HMG Binding Domain; LEF-1; Lymphoid enhancer-binding factor 1; OTTHUMP00000219736; OTTHUMP00000219737; T cell-specific transcription factor 1-alpha; TCF1-alpha

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基因别名: LEF-1; LEF1; TCF10; TCF1ALPHA; TCF7L3

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UniProt ID: (Human) Q9UJU2

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Entrez Gene ID: (Human) 51176

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding beta-catenin binding transcription factor binding DNA binding, bending estrogen receptor activity estrogen receptor binding enhancer binding histone binding histone deacetylase binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process sequence-specific DNA binding transcription regulatory region DNA binding gamma-catenin binding armadillo repeat domain binding C2H2 zinc finger domain binding DNA-binding transcription factor gene-specific transcriptional regulator

参与通路

negative regulation of transcription from RNA polymerase II promoter patterning of blood vessels osteoblast differentiation neural crest cell migration somitogenesis kidney development epithelial to mesenchymal transition sprouting angiogenesis transcription from RNA polymerase II promoter Wnt signaling pathway, calcium modulating pathway positive regulation of cell proliferation response to lithium ion positive regulation of gene expression positive regulation of epithelial to mesenchymal transition Wnt signaling pathway regulation of striated muscle tissue development dentate gyrus development hypothalamus development forebrain radial glial cell differentiation forebrain neuroblast division forebrain neuron differentiation formation of radial glial scaffolds regulation of cell-cell adhesion neutrophil differentiation positive regulation of cell growth embryonic limb morphogenesis positive regulation of cell migration BMP signaling pathway positive regulation of granulocyte differentiation mammary gland development organ regeneration negative regulation of interleukin-13 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production T cell receptor V(D)J recombination B cell proliferation odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA binding steroid hormone mediated signaling pathway tongue development skin development positive regulation by host of viral transcription histone H3 acetylation histone H4 acetylation T-helper 1 cell differentiation negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter alpha-beta T cell differentiation eye pigmentation paraxial mesoderm formation muscle fiber development sensory perception of taste palate development anatomical structure regression canonical Wnt signaling pathway face morphogenesis cell chemotaxis apoptotic process involved in morphogenesis chorio-allantoic fusion trachea gland development cellular response to cytokine stimulus cellular response to interleukin-4 positive regulation of cell proliferation in bone marrow negative regulation of apoptotic process in bone marrow odontoblast differentiation negative regulation of estrogen receptor binding negative regulation of canonical Wnt signaling pathway apoptotic process involved in patterning of blood vessels beta-catenin-TCF complex assembly