OriGene

LHX2 Monoclonal Antibody (OTI5C7), TrueMAB™

查看其他13个LHX2抗体

抗体检测数据 (2)

LHX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LHX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LHX2 Antibody (CF810327) in IHC (P)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA810327)(1:150).

LHX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LHX2 Antibody in Western Blot (WB)

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LHX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA810327)(1:150).

LHX2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX2 (RC210786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX2. (1. Positive lysates LY401508 (100 µg) and LC401508 (20 µg) can be purchased separately from OriGene.

产品信息

CF810327

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

-

免疫组化（石蜡） (IHC (P))

1:150

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI5C7

抗原

Human recombinant protein fragment corresponding to amino acids 220-406 of human LHX2 produced in E.coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Homeobox protein LH-2; LIM homeo box protein 2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2; MGC138390

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基因别名: ap; apterous; hLhx2; Lh-2; LH2; LH2A; LHX2; Lim2

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UniProt ID: (Human) P50458, (Mouse) Q9Z0S2

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Entrez Gene ID: (Human) 9355, (Mouse) 16870, (Rat) 296706

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transcription factor activity, RNA polymerase II transcription factor binding chromatin binding zinc ion binding sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding protein binding metal ion binding helix-turn-helix transcription factor homeodomain transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

参与通路

neural tube closure hair follicle development transcription, DNA-templated axon guidance mesoderm development dorsal/ventral pattern formation olfactory bulb development telencephalon regionalization cerebral cortex development maintenance of epithelial cell apical/basal polarity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter axon extension retina development in camera-type eye negative regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter nervous system development brain development telencephalon development neurogenesis neuron differentiation anatomical structure formation involved in morphogenesis