Invitrogen

LIM1 Polyclonal Antibody

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抗体检测数据 (2)

LIM1 Antibody in Western Blot (WB)

LIM1 Antibody in Western Blot (WB)

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LIM1 Antibody (PA5-78394) in WB

Western blot analysis of LIM1 was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a LIM1 Polyclonal Antibody (Product # PA5-78394) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LIM1 Antibody in Western Blot (WB)

LIM1 Antibody in Western Blot (WB)

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LIM1 Antibody in Western Blot (WB) — Western blot analysis of LIM1 was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a LIM1 Polyclonal Antibody (Product # PA5-78394) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LIM1 Antibody in Western Blot (WB) — Western Blot using LIM1 Polyclonal Antibody (Product # PA5-78394). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 Polyclonal Antibody (Product # PA5-78394) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

产品信息

PA5-78394

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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产品规格

种属反应

Human, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human LIM1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.86 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2736089

产品详细信息

Positive Control: mouse brain, rat brain

Predicted Reactivity: Human (100%), Xenopus laevis (88%), Chicken (84%), Chimpanzee (100%), Bovine (99%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A KO mouse model for the lncRNA Lhx1os produces motor neuron alterations and locomotor impairment.

iScience

Pellegrini F,Padovano V,Biscarini S,Santini T,Setti A,Galfrè SG,Silenzi V,Vitiello E,Mariani D,Nicoletti C,Torromino G,De Leonibus E,Martone J,Bozzoni I

PA5-78394 was used in Western Blotting to describe a conserved motor neuron-specific long non-coding RNA, Lhx1os, whose knockout in mice produces motor impairment and postnatal reduction of mature motor neurons (MNs).

Fri Jan 20 00:00:00 EST 2023

生物信息学

蛋白别名: hLim-1; Homeobox protein Lim-1; LHX-1; LIM homeobox protein 1; LIM/homeobox protein Lhx1; MGC126723; MGC138141; Rlim

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基因别名: LHX1; LIM-1; LIM1

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UniProt ID: (Human) P48742, (Rat) P63007

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Entrez Gene ID: (Human) 3975, (Rat) 257634

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transcription factor activity, sequence-specific DNA binding transcription corepressor activity zinc ion binding sequence-specific DNA binding helix-turn-helix transcription factor homeodomain transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

参与通路

urogenital system development ureteric bud development branching involved in ureteric bud morphogenesis gastrulation with mouth forming second ectoderm formation endoderm formation kidney development transcription from RNA polymerase II promoter cell-cell signaling pattern specification process nervous system development motor neuron axon guidance anatomical structure morphogenesis post-embryonic development embryonic pattern specification organ morphogenesis anterior/posterior axis specification anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression retina layer formation ventral spinal cord development spinal cord association neuron differentiation telencephalon development cerebellum development cerebellar Purkinje cell differentiation forebrain regionalization cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation somite rostral/caudal axis specification metanephric part of ureteric bud development oviduct epithelium development uterine epithelium development nephric duct elongation horizontal cell localization positive regulation of embryonic development cellular response to fibroblast growth factor stimulus negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated anatomical structure formation involved in morphogenesis embryonic viscerocranium morphogenesis pronephros development embryonic retina morphogenesis in camera-type eye uterus development oviduct development cervix development vagina development head development epithelium development paramesonephric duct development comma-shaped body morphogenesis S-shaped body morphogenesis renal vesicle morphogenesis mesonephric duct development nephric duct morphogenesis metanephric glomerulus development metanephric comma-shaped body morphogenesis metanephric renal vesicle morphogenesis metanephric S-shaped body morphogenesis primitive streak formation positive regulation of branching involved in ureteric bud morphogenesis lateral motor column neuron migration positive regulation of gastrulation positive regulation of anterior head development positive regulation of nephron tubule epithelial cell differentiation renal system development mesonephric tubule development