Invitrogen

LIM1 Monoclonal Antibody (OTI2D5)

查看其他25个LIM1抗体

抗体检测数据 (8)

LIM1 Antibody in Immunocytochemistry (ICC/IF)

LIM1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

LIM1 Antibody (MA5-25920) in ICC/IF

Immunofluorescent analysis of LHX1 in COS7 cells. Cells were transfected with a plasmid overexpressing LHX1 and probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunocytochemistry (ICC/IF)

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LIM1 Antibody in Western Blot (WB)

Product Image Gallery

LIM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of LHX1 in COS7 cells. Cells were transfected with a plasmid overexpressing LHX1 and probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LHX1 monoclonal antibody (Product # MA5-25920).

LIM1 Antibody in Western Blot (WB) — Western blot analysis of LHX1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with LHX1 (Product # MA5-25920) monoclonal antibody.

产品信息

MA5-25920

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:150

-

免疫细胞化学 (ICC/IF)

1:100

-

流式细胞分析 (Flow)

1:100

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI2D5

抗原

Human recombinant protein fragment corresponding to amino acids 100-362 of LHX1 produced in HEK293T cell

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.75 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2724601

靶标信息

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: hLim-1; Homeobox protein Lim-1; LHX-1; LIM homeobox protein 1; LIM/homeobox protein Lhx1; MGC126723; MGC138141

查看更多收起

基因别名: LHX1; LIM-1; LIM1

查看更多收起

UniProt ID: (Human) P48742

查看更多收起

Entrez Gene ID: (Human) 3975

查看更多收起

transcription factor activity, sequence-specific DNA binding transcription corepressor activity zinc ion binding sequence-specific DNA binding helix-turn-helix transcription factor homeodomain transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

参与通路

urogenital system development ureteric bud development branching involved in ureteric bud morphogenesis gastrulation with mouth forming second ectoderm formation endoderm formation kidney development transcription from RNA polymerase II promoter cell-cell signaling pattern specification process nervous system development motor neuron axon guidance anatomical structure morphogenesis post-embryonic development embryonic pattern specification organ morphogenesis anterior/posterior axis specification anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression retina layer formation ventral spinal cord development spinal cord association neuron differentiation telencephalon development cerebellum development cerebellar Purkinje cell differentiation forebrain regionalization cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation somite rostral/caudal axis specification metanephric part of ureteric bud development oviduct epithelium development uterine epithelium development nephric duct elongation horizontal cell localization positive regulation of embryonic development cellular response to fibroblast growth factor stimulus negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated anatomical structure formation involved in morphogenesis embryonic viscerocranium morphogenesis pronephros development embryonic retina morphogenesis in camera-type eye uterus development oviduct development cervix development vagina development head development epithelium development paramesonephric duct development comma-shaped body morphogenesis S-shaped body morphogenesis renal vesicle morphogenesis mesonephric duct development nephric duct morphogenesis metanephric glomerulus development metanephric comma-shaped body morphogenesis metanephric renal vesicle morphogenesis metanephric S-shaped body morphogenesis primitive streak formation positive regulation of branching involved in ureteric bud morphogenesis lateral motor column neuron migration positive regulation of gastrulation positive regulation of anterior head development positive regulation of nephron tubule epithelial cell differentiation