Invitrogen

LIME Polyclonal Antibody

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LIME Antibody (PA1-9064) in WB

Western blot analysis of LIME using LIME Polyclonal Antibody (Product # PA1-9064) (1 µg/mL) in staining of Mouse Heart lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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产品信息

PA1-9064

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to amino acids ENVYESIKEMGL.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2136804

产品详细信息

This antibody is predicted to react with rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

靶标信息

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。