Invitrogen

LRP1 Monoclonal Antibody (A2MR-beta-1)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

9个已发表图片

12 篇参考文献

查看其他38个LRP1 (CD91)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

LRP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

LRP1 Antibody (37-7600) in ICC/IF

Immunofluorescence analysis of LRP1 was performed using 70% confluent log phase U-87 MG and SK-BR-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 2 hours at room temperature. The cells were labeled with LRP1 Monoclonal Antibody (A2MR-beta-1) (Product # 37-7600) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488... View More

LRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LRP1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

LRP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

LRP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using LRP1 Monoclonal Antibody (A2MR-beta-1) (Product # 37-7600) shows expression of LRP1 in U-87-MG cell line in comparison to SK-BR-3. {RE}

产品信息

37-7600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:1,000

免疫组化（冰冻） (IHC (F))

1:1,000

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

A2MR-beta-1

抗原

Synthetic peptide derived from the human CD91 beta chain.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533340

产品详细信息

37-7600 was used in IHC to successfully detect LRP-1 in mouse E18.5 lung section.

靶标信息

The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

37-7600 was used in Western Blotting and Immunoprecipitaiton to identify cell type-specific extracellular vesicle (EV) protein markers in human-induced pluripotent stem cell-derived neural cell types, including excitatory neurons (ATP1A3, NCAM1), astrocytes (LRP1, ITGA6), microglia-like cells (ITGAM, LCP1), and oligodendrocyte-like cells (LAMP2, FTH1), along with 16 pan-EV marker candidates.

Sat Jan 01 00:00:00 EST 2022

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

Sat Jan 01 00:00:00 EST 2022

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

Sat Jan 01 00:00:00 EST 2022

ApoE3 vs. ApoE4 Astrocytes: A Detailed Analysis Provides New Insights into Differences in Cholesterol Homeostasis.

Antioxidants (Basel, Switzerland)

Staurenghi E,Leoni V,Lo Iacono M,Sottero B,Testa G,Giannelli S,Leonarduzzi G,Gamba P

37-7600 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to find that ApoE genotype clearly affects astrocytic cholesterol homeostasis.

Tue Nov 01 00:00:00 EDT 2022

LRP-1 receptor combines EGFR signalling and eHsp90α autocrine to support constitutive breast cancer cell motility in absence of blood supply.

Scientific reports

Chang C,Tang X,Mosallaei D,Chen M,Woodley DT,Schönthal AH,Li W

37-7600 was used in Western Blotting to uncover a novel mechanism by which certain breast cancer cells use lipoprotein receptor-related protein 1 to engage parallel signalling pathways to move when they lose contact with blood support.

Thu Jul 14 00:00:00 EDT 2022

Bio-nanoparticle based therapeutic vaccine induces immunogenic response against triple negative breast cancer.

American journal of cancer research

Bai X,Zhou Y,Lin Q,Huang CK,Zhang S,Carlson RI,Ghanbari H,Sun B,Wands JR,Dong X

37-7600 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to conclude that λ phage vaccine targeting a TAA (ASPH) combined with DOX successfully induces ICD and initiates CD8+ T cell-mediated antitumor immunity in breast tumors.

Fri Oct 22 00:00:00 EDT 2021

Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Scientific reports

Dong H,Zou M,Bhatia A,Jayaprakash P,Hofman F,Ying Q,Chen M,Woodley DT,Li W

Published figure using LRP1 monoclonal antibody (Product # 37-7600) in Western Blot

Fri Feb 05 00:00:00 EST 2016

Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Scientific reports

Dong H,Zou M,Bhatia A,Jayaprakash P,Hofman F,Ying Q,Chen M,Woodley DT,Li W

Published figure using LRP1 monoclonal antibody (Product # 37-7600) in Western Blot

Fri Feb 05 00:00:00 EST 2016

Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Journal of cell science

Jayaprakash P,Dong H,Zou M,Bhatia A,O'Brien K,Chen M,Woodley DT,Li W

37-7600 was used in western blot to elucidate a role for heat shock proteins in ischemia.

Wed Apr 15 00:00:00 EDT 2015

Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Journal of cell science

Jayaprakash P,Dong H,Zou M,Bhatia A,O'Brien K,Chen M,Woodley DT,Li W

37-7600 was used in western blot to elucidate a role for heat shock proteins in ischemia.

Wed Apr 15 00:00:00 EDT 2015

Extracellular heat shock protein 90 signals through subdomain II and the NPVY motif of LRP-1 receptor to Akt1 and Akt2: a circuit essential for promoting skin cell migration in vitro and wound healing in vivo.

Molecular and cellular biology

Tsen F,Bhatia A,O'Brien K,Cheng CF,Chen M,Hay N,Stiles B,Woodley DT,Li W

37-7600 was used in immunoprecipitation and western blot to elucidate the mechanism by which eHsp90α regulates promotility action.

Sun Dec 01 00:00:00 EST 2013

Molecular and cellular biology

Tsen F,Bhatia A,O'Brien K,Cheng CF,Chen M,Hay N,Stiles B,Woodley DT,Li W

37-7600 was used in immunoprecipitation and western blot to elucidate the mechanism by which eHsp90α regulates promotility action.

Sun Dec 01 00:00:00 EST 2013

Molecular and cellular biology

Tsen F,Bhatia A,O'Brien K,Cheng CF,Chen M,Hay N,Stiles B,Woodley DT,Li W

37-7600 was used in immunoprecipitation and western blot to elucidate the mechanism by which eHsp90α regulates promotility action.

Sun Dec 01 00:00:00 EST 2013

生物信息学

蛋白别名: A2MR; alpha 2-macroglobulin receptor; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; CD91; FLJ16451; lipoprotein receptor-related protein; low density lipoprotein receptor-related protein 1; low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor); LRP-1; lrp1; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; type V tgf-beta receptor

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基因别名: A2MR; AI316852; APOER; APR; b2b1554Clo; CD91; IGFBP3R; LRP; LRP1; LRP1A; TGFBR5

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UniProt ID: (Human) Q07954, (Mouse) Q91ZX7, (Rat) G3V928

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Entrez Gene ID: (Human) 4035, (Mouse) 16971, (Rat) 299858

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功能