Invitrogen

LSD1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片
3 篇参考文献
查看其他26个LSD1抗体
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说明书
实验方案
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产品信息

PA5-17361

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

-
查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:400
-

流式细胞分析 (Flow)

1:400
-

免疫沉淀 (IP)

1:50
-

染色质免疫沉淀 实验 (ChIP)

5 µg
-
产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

19.6 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10982296

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; neuroprotective protein 3; RP1-184J9.1

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基因别名: 1810043O07Rik; AA408884; AOF2; BHC110; CPRF; D4Ertd478e; KDM1; KDM1A; KIAA0601; LSD1; mKIAA0601; RGD1562975

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UniProt ID: (Human) O60341, (Mouse) Q6ZQ88

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Entrez Gene ID: (Human) 23028, (Mouse) 99982, (Rat) 500569

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功能
RNA polymerase II transcription factor binding p53 binding chromatin binding transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding oxidoreductase activity enzyme binding ligand-dependent nuclear receptor transcription coactivator activity demethylase activity histone demethylase activity histone demethylase activity (H3-K4 specific) histone demethylase activity (H3-K9 specific) histone demethylase activity (H3-dimethyl-K4 specific) telomeric DNA binding MRF binding transcription regulatory region DNA binding flavin adenine dinucleotide binding androgen receptor binding telomeric repeat-containing RNA binding DNA binding N-ethylmaleimide reductase activity reduced coenzyme F420 dehydrogenase activity sulfur oxygenase reductase activity malolactic enzyme activity NADPH:sulfur oxidoreductase activity epoxyqueuosine reductase activity oxidase
参与通路
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein demethylation blood coagulation cell proliferation regulation of double-strand break repair via homologous recombination regulation of primitive erythrocyte differentiation histone deacetylation pituitary gland development granulocyte differentiation negative regulation of protein binding histone H3-K9 demethylation positive regulation of histone ubiquitination cellular response to UV histone H3-K4 demethylation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of erythrocyte differentiation positive regulation of megakaryocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone biosynthetic process positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation muscle cell development oxidation-reduction process cellular response to gamma radiation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of cellular protein localization positive regulation of neural precursor cell proliferation positive regulation of stem cell proliferation regulation of transcription, DNA-templated multicellular organism development positive regulation of neuron projection development chromatin modification positive regulation of chromatin binding positive regulation of cell size guanine metabolic process negative regulation of transcription from RNA polymerase II promoter by histone modification alternative mRNA splicing, via spliceosome response to organic cyclic compound cerebral cortex development neuron maturation response to fungicide cellular response to cAMP
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