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LXR alpha Antibody (MA5-32691) in ICC/IF
Immunocytochemical analysis of LXR alpha in HepG2 cells using a LXR alpha Monoclonal antibody (Product # MA5-32691) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
产品信息
MA5-32691
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JA20-38
抗原
Synthetic peptide within Human LXR alpha aa 49-98
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809968
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Its interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. NR1H3 plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. It also interplays functionally with RORA for the regulation of genes involves in liver metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: liver X nuclear receptor alpha variant 1; Liver X receptor alpha; LXR alpha; LXRb; NER; NERI; NR1H2; nuclear orphan receptor LXR-alpha; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; Oxysterols receptor LXR-alpha; RLD-1; ubiquitously-expressed nuclear receptor 1
基因别名: AU018371; LXR; LXR-a; LXRA; LXRalpha; NR1H3; RLD-1; RLD1; Unr1
UniProt ID: (Human) Q13133, (Mouse) Q9Z0Y9
Entrez Gene ID: (Human) 10062, (Mouse) 22259, (Rat) 58852
DNA binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
cholesterol binding
sterol response element binding
sequence-specific DNA binding
transcription regulatory region DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
metal ion binding
retinoid X receptor binding
protein heterodimerization activity
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
fatty acid biosynthetic process
positive regulation of triglyceride biosynthetic process
positive regulation of receptor biosynthetic process
positive regulation of cholesterol efflux
negative regulation of cholesterol storage
intracellular receptor signaling pathway
positive regulation of cellular protein metabolic process
negative regulation of lipid transport
positive regulation of cholesterol transport
response to progesterone
positive regulation of toll-like receptor 4 signaling pathway
cholesterol homeostasis
negative regulation of macrophage activation
apoptotic cell clearance
steroid hormone mediated signaling pathway
cellular lipid metabolic process
positive regulation of fatty acid biosynthetic process
negative regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of pinocytosis
negative regulation of inflammatory response
positive regulation of lipoprotein lipase activity
lipid homeostasis
sterol homeostasis
triglyceride homeostasis
cellular response to lipopolysaccharide
negative regulation of pancreatic juice secretion
negative regulation of secretion of lysosomal enzymes
regulation of cholesterol homeostasis
positive regulation of cholesterol homeostasis
transcription initiation from RNA polymerase II promoter
negative regulation of macrophage derived foam cell differentiation
regulation of circadian rhythm
negative regulation of interferon-gamma-mediated signaling pathway
lipid metabolic process
insulin receptor signaling pathway
