Invitrogen

Lambda light chain Monoclonal Antibody (36-59)

Lambda light chain Antibody in Western Blot (WB)

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Lambda light chain Antibody (MA5-44638) in WB

Western blot analysis of Human Lambda light chain in human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in Human Lambda light chain Monoclonal antibody (Product # MA5-44638) using a dilution of 1:1,000 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:5,000 for 1 hour at room temperature.

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产品信息

MA5-44638

应用

建议稀释比

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免疫印迹 (WB)

1:1,000

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

36-59

抗原

Synthetic peptide within human lambda light chain aa 1-106.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2931098

产品详细信息

Application notes: Indirect ELISA

靶标信息

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。