Invitrogen

Laminin beta-1 Monoclonal Antibody (LT3)

Laminin beta-1 Antibody in Western Blot (WB)

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Laminin beta-1 Antibody (MA5-14657) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Heart (Lane 3), Rat heart (Lane 4), Mouse Stomach (Lane 5) and Mouse Spleen (Lane 6). The blot was probed with Anti-Laminin beta-1 Monoclonal Antibody (LT3) (Product # MA5-14657, 1µg/ml dilution) and detected by chemiluminescence using F(ab')2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP conjugate (Product # PA1-29927, 0.25 µg/ml, 1:4000 dilution). A 200 kDa band corresponding to Laminin beta-1 beta was observed acr... View More

Laminin beta-1 Antibody in Immunoprecipitation (IP)

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产品信息

MA5-14657

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

- -

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

2 µg/mL

免疫组化（冰冻） (IHC (F))

2 µg/mL

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

2 µg/mL

产品规格

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

LT3

抗原

Murine EHS laminin preparation

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10981503

产品详细信息

MA5-14657 targets Laminin B1/Beta1 in IF, IHC (P, F), IP, and WB applications and shows reactivity with Hamster, Human, mouse, and Porcine samples.

The MA5-14657 immunogen is mouse EHS laminin preparation.

靶标信息

Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca²⁺ signalling to control glucose-dependent insulin secretion.

eLife

Jevon D,Deng K,Hallahan N,Kumar K,Tong J,Gan WJ,Tran C,Bilek M,Thorn P

MA5-14657 was used in Immunocytochemistry-immunoflourescence to conclude that FAK orchestrates the final steps of glucose-dependent insulin secretion within the restricted domain where β-cell contact the islet capillaries.

Fri May 13 00:00:00 EDT 2022

The Rhesus Macaque Serves As a Model for Human Lateral Branch Nephrogenesis.

Journal of the American Society of Nephrology : JASN

Schuh MP,Alkhudairy L,Potter A,Potter SS,Chetal K,Thakkar K,Salomonis N,Kopan R

MA5-14657 was used in Immunohistochemistry to understand lateral branch nephrogenesis (LBN) at the molecular level.

Mon May 03 00:00:00 EDT 2021

Reduced synchroneity of intra-islet Ca²⁺ oscillations in vivo in Robo-deficient β cells.

eLife

Adams MT,Dwulet JM,Briggs JK,Reissaus CA,Jin E,Szulczewski JM,Lyman MR,Sdao SM,Kravets V,Nimkulrat SD,Ponik SM,Merrins MJ,Mirmira RG,Linnemann AK,Benninger RK,Blum B

MA5-14657 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to provide evidence that this loss is not due to a β cell-intrinsic function of Robo, mis-expression or mis-localization of Cx36 gap junctions, or changes in islet vascularization or innervation, suggesting that the islet architecture itself is required for synchronized Ca2+ oscillations.

Wed Jul 07 00:00:00 EDT 2021

p120-catenin-dependent collective brain infiltration by glioma cell networks.

Nature cell biology

Gritsenko PG,Atlasy N,Dieteren CEJ,Navis AC,Venhuizen JH,Veelken C,Schubert D,Acker-Palmer A,Westerman BA,Wurdinger T,Leenders W,Wesseling P,Stunnenberg HG,Friedl P

MA5-14657 was used in Immunocytochemistry-Immunofluorescence to study organotypic three-dimensional (3D) culture and in vivo analyses and identify the collective network invasion deployed by glioma cells.

Wed Jan 01 00:00:00 EST 2020

Whole-Exome Sequencing in Age-Related Macular Degeneration Identifies Rare Variants in COL8A1, a Component of Bruch's Membrane.

Ophthalmology

Corominas J,Colijn JM,Geerlings MJ,Pauper M,Bakker B,Amin N,Lores Motta L,Kersten E,Garanto A,Verlouw JAM,van Rooij JGJ,Kraaij R,de Jong PTVM,Hofman A,Vingerling JR,Schick T,Fauser S,de Jong EK,van Duijn CM,Hoyng CB,Klaver CCW,den Hollander AI

MA5-14657 was used in Immunohistochemistry-immunofluorescence to study a role for protein-altering variants in the COL8A1 gene in age-related macular degeneration pathogenesis.

Sat Sep 01 00:00:00 EDT 2018

Deletion of the laminin alpha4 chain leads to impaired microvessel maturation.

Molecular and cellular biology

Thyboll J,Kortesmaa J,Cao R,Soininen R,Wang L,Iivanainen A,Sorokin L,Risling M,Cao Y,Tryggvason K

MA5-14657 was used in immunohistochemistry to study the essential role of laminin alpha4 chain in microvessel maturation

Fri Feb 01 00:00:00 EST 2002

Laminin beta-1 Monoclonal Antibody (LT3)

Laminin beta-1 Antibody (MA5-14657) in WB

产品信息

MA5-14657

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

性能保障

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