Invitrogen

Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), PE, eBioscience™

Ly-108 Antibody in Flow Cytometry (Flow)

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Ly-108 Antibody (12-1508-80) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or 0.06 µg of Anti-Mouse Ly-108 PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-1508-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio13G3-19D (13G3-19D)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_763616

产品详细信息

Description: The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.

Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Applications Reported: This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Ly108 is the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is expressed on NK, B cells, and T cells. Activation of Ly108 receptor enhances NK cell activity and IFN-gamma expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

LSD1 inhibition sustains T cell invigoration with a durable response to PD-1 blockade.

Nature communications

Liu Y,Debo B,Li M,Shi Z,Sheng W,Shi Y

12-1508-80 was used in Flow Cytometry to demonstrate that histone demethylase LSD1 acts to enforce an epigenetic program in progenitor exhausted CD8+ T cells to antagonize the TCF1-mediated progenitor maintenance and to promote terminal differentiation.

Wed Nov 24 00:00:00 EST 2021

T/B-cell interactions are more transient in response to weak stimuli in SLE-prone mice.

European journal of immunology

Sinai P,Dozmorov IM,Song R,Schwartzberg PL,Wakeland EK,Wülfing C

12-1508 was used in Flow cytometry/Cell sorting to analyse primary T and B cells from a mouse model of systemic lupus erythematosus prior to the onset of disease.