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Ly-6E Antibody (PA5-143999) in IHC (P)
Immunohistochemistry analysis of Ly-6E in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with Ly-6E Polyclonal antibody (Product # PA5-143999) using a dilution of 2 μg/mL overnight at 4°C. Biotinylated goat ant... View More
产品信息
PA5-143999
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human LY6E/SCA-2 recombinant protein (Position: L21-S101).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_3075213
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
靶标信息
Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: locus A/E; Locus AE; Ly-6E; Ly6A; Lymphocyte antigen 6E; retinoic acid induced gene E; Retinoic acid-induced gene E protein; RIG-E; SCA-2; Stem cell antigen 2; Thymic shared antigen 1; TSA-1
基因别名: 9804; LY6E; RIG-E; RIGE; SCA-2; SCA2; TSA-1; TSA1
UniProt ID: (Human) Q16553
Entrez Gene ID: (Human) 4061
