Invitrogen

M-CSF Polyclonal Antibody

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M-CSF Antibody (PA5-95548) in WB

Western blot analysis of M-CSF in Lane 1: human placenta tissue lysates, Lane 2: human 293T whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with M-CSF polyclonal antibody (Product # PA5-95548) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescen... View More

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产品信息

PA5-95548

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human CSF1 recombinant protein (Position: E33-N190).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2807350

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor

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基因别名: C87615; CSF-1; CSF1; Csfm; MCSF; op

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UniProt ID: (Human) P09603, (Mouse) P07141

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Entrez Gene ID: (Human) 1435, (Mouse) 12977

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功能

cytokine activity macrophage colony-stimulating factor receptor binding protein binding growth factor activity protein homodimerization activity

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