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Description: The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50-60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and is a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in homing of subpopulation of memory T cells to the gut lamina propria, and recirculation of naive lymphocytes in Peyer's patches and mesenteric lymph nodes.
Applications Reported: MECA-367 can be used in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, blocking in adhesion assays, immunoprecipitation and immunoblotting (WB).
Applications Tested: The MECA-367 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: MAdCAM-1; mMAdCAM-1; mucosal addressin cell adhesion molecule 1
基因别名: AV211525
Entrez Gene ID: (Mouse) 17123