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Invitrogen
MBD1 Recombinant Rabbit Monoclonal Antibody (12H8L4)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 5
MBD1 Antibody (703370) in ICC/IF
For immunocytochemistry analysis, HeLa cells were fixed and permeabilized for detection of endogenous MBD1 using Anti-MBD1 Recombinant Rabbit Monoclonal Antibody (Product # 703370, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of MBD1 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product... View More
产品信息
703370
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
12H8L4
抗原
Protein corresponding to human MBD1 [aa400-aa605]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809233
产品详细信息
This antibody is predicted to react with Monkey, Bovine.
靶标信息
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CXXC-type zinc finger protein 3; methyl-CpG binding domain protein 1 isoform PCM1; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1; the regulator of fibroblast growth factor 2 (FGF-2) transcription
基因别名: CXXC3; MBD1; PCM1; RFT
UniProt ID: (Human) O95241
Entrez Gene ID: (Human) 4152
negative regulation of transcription from RNA polymerase II promoter
methylation-dependent chromatin silencing
transcription from RNA polymerase II promoter
heart development
aging
response to fluoxetine
neuron differentiation
response to nutrient levels
response to estradiol
response to cocaine
regulation of DNA methylation
negative regulation of transcription, DNA-templated
negative regulation of astrocyte differentiation
