Invitrogen

MBP Polyclonal Antibody

MBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MBP Antibody (PA5-78397) in IHC (P)

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Myelin basic Protein stained by Myelin basic Protein antibody (Product # PA5-78397) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

MBP Antibody in Immunohistochemistry (Frozen) (IHC (F))

MBP Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-78397

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:10,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Myelin Basic Protein. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2736178

产品详细信息

Positive Control: mouse brain, rat brain

Predicted reactivity: Mouse (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Gonadotropin-releasing hormone and growth hormone act as anti-inflammatory factors improving sensory recovery in female rats with thoracic spinal cord injury.

Frontiers in neuroscience

Martínez-Moreno CG,Calderón-Vallejo D,Díaz-Galindo C,Hernández-Jasso I,Olivares-Hernández JD,Ávila-Mendoza J,Epardo D,Balderas-Márquez JE,Urban-Sosa VA,Baltazar-Lara R,Carranza M,Luna M,Arámburo C,Quintanar JL

PA5-78397 was used in Immunohistochemistry-immunofluorescence to provide evidence of an anti-inflammatory and glial-modulatory effect of GnRH and GH in an experimental model of SCI and suggest that these hormones can modulate the response of microglia, astrocytes, and infiltrated immune cells in the spinal cord tissue following injury.

Sat Jul 01 00:00:00 EDT 2023

Gonadotropin-releasing hormone and growth hormone act as anti-inflammatory factors improving sensory recovery in female rats with thoracic spinal cord injury.

Frontiers in neuroscience

Sat Jul 01 00:00:00 EDT 2023

Potential Role of Intracranial Mast Cells in Neuroinflammation and Neuropathology Associated with Food Allergy.

Cells

Germundson DL,Nagamoto-Combs K

PA5-78397 was used in Western Blot to support the ability of brain MCs to release histamine and other mediators to increase BBB permeability and facilitate neuroinflammatory responses in the brain.

Sun Feb 20 00:00:00 EST 2022

Potential Role of Intracranial Mast Cells in Neuroinflammation and Neuropathology Associated with Food Allergy.

Cells

Germundson DL,Nagamoto-Combs K

PA5-78397 was used in Western Blot to support the ability of brain MCs to release histamine and other mediators to increase BBB permeability and facilitate neuroinflammatory responses in the brain.

Sun Feb 20 00:00:00 EST 2022

Gallic and ascorbic acids supplementation alleviate cognitive deficits and neuropathological damage exerted by cadmium chloride in Wistar rats.

Scientific reports

Adebiyi O,Adigun K,David-Odewumi P,Akindele U,Olayemi F

Published figure using MBP polyclonal antibody (Product # PA5-78397) in Immunohistochemistry

Wed Aug 24 00:00:00 EDT 2022

Cerebellar demyelination and neurodegeneration associated with mTORC1 hyperactivity may contribute to the developmental onset of autism-like neurobehavioral phenotype in a rat model.

Autism research : official journal of the International Society for Autism Research

Kútna V,O'Leary VB,Hoschl C,Ovsepian SV

PA5-78397 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry to elucidate the impact of constitutive mTORC1 hyperactivity on cerebellar morphology and cellular organization in a rat model of autism and epilepsy.

Sun May 01 00:00:00 EDT 2022

Ciclesonide activates glucocorticoid signaling in neonatal rat lung but does not trigger adverse effects in the cortex and cerebellum.

Neurobiology of disease

Jaumotte JD,Franks AL,Bargerstock EM,Kisanga EP,Menden HL,Ghersi A,Omar M,Wang L,Rudine A,Short KL,Silswal N,Cole TJ,Sampath V,Monaghan-Nichols AP,DeFranco DB

PA5-78397 was used in Immunohistochemistry to conclude that CIC is a promising, novel candidate drug to treat or prevent BPD in neonates given its activation of GR in neonatal lung and limited adverse neurodevelopmental effects.

Sun Aug 01 00:00:00 EDT 2021

生物信息学

蛋白别名: golli mbp; Golli-Mbp; myelin basic protein; Golli-Mbp; myelin basic protein; myelin basic protein S; MBP; MBP S; MGC99675; Myelin A1 protein; Myelin basic protein; myelin basic protein Golli-mbp; myelin basic protein S; myelin deficient; Myelin membrane encephalitogenic protein; shiverer

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基因别名: C76307; golli-mbp; Hmbpr; MBP; Mbps; mld; R75289; Shi

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UniProt ID: (Human) P02686, (Rat) P02688, (Mouse) P04370

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Entrez Gene ID: (Human) 4155, (Rat) 24547, (Mouse) 17196

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功能

protease binding protein binding structural constituent of myelin sheath myelin protein structural protein

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