Invitrogen

MC4R Polyclonal Antibody

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MC4R Antibody (PA5-72246) in WB

Western blot analysis of MC4R in various lysates. Samples were incubated with MC4R polyclonal antibody (Product # PA5-72246) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: ZF4 whole cell lysate; Lane 2: Zebrafish whole cell lysate; Lane 3: Z. muscle whole cell lysate. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

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产品信息

PA5-72246

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

产品规格

种属反应

Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 25-57 amino acids from the human region of DANRE mc4r

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2718100

靶标信息

Expression of MC4R has been reported in brain. Unlike the other melanocortin receptors, MC4R is not expressed in adrenal cortex or in melanocytes. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.

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