Invitrogen

MDC Polyclonal Antibody, PeproTech®

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MDC Antibody (500-P107-1MG) in WB

Western Blot: To detect Human MDC (CCL22) by Western Blot analysis MDC Polyclonal Antibody (Product # 500-P107-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P107-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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中和实验 (Neu)

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体外检测 (IV)

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human MDC (CCL22) (69 a.a.)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929223

产品详细信息

AA Sequence of recombinant protein: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MDC (CCL22). Anti-Human MDC (CCL22)-specific antibody was purified by affinity chromatography employing an immobilized Human MDC (CCL22) matrix.

Sandwich ELISA: To detect Human MDC (CCL22) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MDC (CCL22) (500-P107Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MDC (CCL22).

Western Blot: To detect Human MDC (CCL22) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Also, it is a chemotactic for monocytes. dendritic cells and natural killer cells. CCL22 is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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500-P107 was used in Immunohistochemistry to study the recruitment of regulatory T cells (Tregs) which are key players in processes mediating immune tolerance and identify galectin-2 (Gal-2) as a factor that seems to prevent the apoptosis of Tregs.

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Soluble factors from T cells inhibiting X4 strains of HIV are a mixture of β chemokines and RNases.

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Diverse and potent chemokine production by lung CD11bhigh dendritic cells in homeostasis and in allergic lung inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Beaty SR,Rose CE,Sung SS

Thu Feb 01 00:00:00 UTC 2007

Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.

Blood

Cattoretti G,Büttner M,Shaknovich R,Kremmer E,Alobeid B,Niedobitek G

Mon May 15 00:00:00 UTC 2006

Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.

Journal of immunology (Baltimore, Md. : 1950)

Mateo T,Abu Nabah YN,Abu Taha M,Mata M,Cerdá-Nicolás M,Proudfoot AE,Stahl RA,Issekutz AC,Cortijo J,Morcillo EJ,Jose PJ,Sanz MJ

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Expression of CCL28 by Reed-Sternberg cells defines a major subtype of classical Hodgkin's disease with frequent infiltration of eosinophils and/or plasma cells.

The American journal of pathology

Hanamoto H,Nakayama T,Miyazato H,Takegawa S,Hieshima K,Tatsumi Y,Kanamaru A,Yoshie O

Mon Mar 01 00:00:00 UTC 2004

Macrophage-derived chemokine (MDC/CCL22) and CCR4 are involved in the formation of T lymphocyte-dendritic cell clusters in human inflamed skin and secondary lymphoid tissue.

The American journal of pathology

Katou F,Ohtani H,Nakayama T,Ono K,Matsushima K,Saaristo A,Nagura H,Yoshie O,Motegi K

Sun Apr 01 00:00:00 UTC 2001

生物信息学

蛋白别名: AW060611; C-C motif chemokine 22; CC chemokine STCP-1; chemokine (C-C motif) ligand 22; Macrophage-derived chemokine; MDC(1-69); MGC108943; MGC34554; RP24-69A20.1; small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; stimulated T cell chemotactic protein 1; Stimulated T-cell chemotactic protein 1

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基因别名: A-152E5.1; ABCD-1; CCL22; DC/B-CK; MDC; SCYA22; STCP-1

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UniProt ID: (Human) O00626

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Entrez Gene ID: (Human) 6367

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功能

chemokine activity CCR chemokine receptor binding cytokine intercellular signal molecule

参与通路