Invitrogen

MECP2 Monoclonal Antibody (5H12)

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MECP2 Antibody in Immunohistochemistry (IHC)

MECP2 Antibody in Immunohistochemistry (IHC)

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MECP2 Antibody (MA5-33096) in IHC

Immunohistochemistry analysis of MECP2 in rat cerebellum. Showing specific labeling of MECP2 monoclonal antibody (Product # MA5-33096) using a dilution of 1:1,000 (red) in nuclei of neurons and specific labeling of GFAP with a dilution of 1:5,000 (green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.

MECP2 Antibody in Immunohistochemistry (IHC)

MECP2 Antibody in Immunohistochemistry (IHC)

MECP2 Antibody in Immunohistochemistry (IHC)

MECP2 Antibody in Western Blot (WB)

MECP2 Antibody in Immunocytochemistry (ICC/IF)

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MECP2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of MECP2 in rat cerebellum. Showing specific labeling of MECP2 monoclonal antibody (Product # MA5-33096) using a dilution of 1:1,000 (red) in nuclei of neurons and specific labeling of GFAP with a dilution of 1:5,000 (green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.

MECP2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of MECP2 in rat cerebellum labeled with MECP2 monoclonal antibody (Product # MA5-33096) using a dilution of 1:500 (red), colabeled with anti-GFAP using a dilution of 1:5,000 (green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

MECP2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of MECP2 in a section of rat hippocampus showing specific nuclear immunolabeling of MECP2 monoclonal antibody (Product # MA5-33096) using a dilution of 1:1,000 (red) and FOX3 (green). The blue is DAPI staining of nuclear DNA.

MECP2 Antibody in Western Blot (WB) — Western blot of MECP2 in mouse whole brain lysate showing specific immunolabeling of a MECP2 monoclonal antibody (Product # MA5-33096) at a band of ~75 kDa.

MECP2 Antibody in Immunocytochemistry (ICC/IF) — Generation of isogenic iPSC-derived cardiomyocytes expressing different MeCP2 alleles. ( A ) The MeCP2 mutation is indicated by the arrow head, and the expression of specific MeCP2 allele in the isogenic iPSCs was confirmed by PCR analysis. ( B ) Immunostaining analysis of the isogenic iPSCs with MECP2-specific antibody (ThermoFisher, Waltham, MA, USA, MA5-33096) indicated the nucleic localization of wildtype MECP2 protein. ( C ) The iPSC lines were differentiated into cardiomyocytes (iPSC-CMs) and co-stained with cardiac troponin T (green, Abcam, Cambridge, UK, ab45932) and sarcomeric alpha-actinin (red, sigma, A7811), and the nuclei were stained with DAPI (blue). ( D ) The cell size of the iPSC-CMs. N = 284, 357, and 360 cells measured from normal, MeCP2 wildtype , and MeCP2 mutant groups, respectively. Data are represented as mean +- SEM. Scale bar: 100 um. (See also Figure S1 ).

产品信息

MA5-33096

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

-

免疫组化 (IHC)

1:500-1:5,000

-

免疫细胞化学 (ICC/IF)

1:1,000-1:5,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

5H12

抗原

Full length human recombinant MeCP2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

~1 mg/mL

纯化类型

Protein G

保存液

PBS with 50% glycerol

内含物

5mM sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2802646

靶标信息

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Isogenic Human-Induced Pluripotent Stem-Cell-Derived Cardiomyocytes Reveal Activation of Wnt Signaling Pathways Underlying Intrinsic Cardiac Abnormalities in Rett Syndrome.

International journal of molecular sciences

Ng KM,Ding Q,Tse YL,Chou OH,Lai WH,Au KW,Lau YM,Ji Y,Siu CW,Tang CS,Colman A,Tsang SY,Tse HF

Published figure using MECP2 monoclonal antibody (Product # MA5-33096) in Immunocytochemistry

Fri Dec 09 00:00:00 EST 2022

生物信息学

蛋白别名: DKFZp686A24160; MeCp-2 protein; methyl CpG binding protein 2; Methyl-CpG-binding protein 2; testis tissue sperm-binding protein Li 41a

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基因别名: 1500041B07Rik; AUTSX3; D630021H01Rik; Mbd5; MECP2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP10

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UniProt ID: (Human) P51608, (Mouse) Q9Z2D6, (Rat) Q00566

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Entrez Gene ID: (Human) 4204, (Mouse) 17257, (Rat) 29386

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four-way junction DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity mRNA binding protein binding transcription factor binding methyl-CpG binding double-stranded methylated DNA binding protein domain specific binding chromatin DNA binding siRNA binding histone deacetylase binding poly(A) RNA binding unmethylated CpG binding protein N-terminus binding chromatin binding enzyme binding DNA metabolism protein

参与通路

negative regulation of transcription from RNA polymerase II promoter behavioral fear response response to hypoxia startle response neurological system process involved in regulation of systemic arterial blood pressure regulation of respiratory gaseous exchange by neurological system process inositol metabolic process mitochondrial electron transport, ubiquinol to cytochrome c chromatin silencing regulation of gene expression by genetic imprinting transcription, DNA-templated glutamine metabolic process cellular biogenic amine metabolic process synapse assembly heart development respiratory gaseous exchange long-term memory locomotory behavior protein localization glucocorticoid metabolic process positive regulation of cell proliferation adult locomotory behavior visual learning response to radiation pathogenesis post-embryonic development response to ionizing radiation response to lead ion dendrite development histone methylation histone acetylation proprioception sensory perception of pain cerebellum development ventricular system development negative regulation of histone methylation cardiolipin metabolic process response to estradiol negative regulation of histone acetylation social behavior neuron maturation negative regulation of neuron apoptotic process regulation of DNA methylation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated phosphatidylcholine metabolic process negative regulation of astrocyte differentiation catecholamine secretion positive regulation of synapse assembly excitatory postsynaptic potential long-term synaptic potentiation regulation of transcription, DNA-templated chemical synaptic transmission brain development learning memory embryo development regulation of gene expression neuron differentiation neuron projection development multicellular organismal response to stress regulation of gene expression, epigenetic regulation of synaptic plasticity neuromuscular process controlling posture neuromuscular process response to organic cyclic compound