Invitrogen

MED14 Polyclonal Antibody

MED14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MED14 Antibody (PA5-99205) in IHC (P)

Immunohistochemistry analysis of paraffin-embedded MED14 in human brain tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with MED14 polyclonal antibody (Product # PA5-99205) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

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产品信息

PA5-99205

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MED14(Accession O60244), corresponding to amino acid residues L1134-I1184.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2818138

产品详细信息

Antibody detects endogenous levels of total MED14.

靶标信息

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; cofactor required for Sp1 transcriptional activation subunit 2 (150 kDa); cofactor required for Sp1 transcriptional activation, subunit 2; cofactor required for Sp1 transcriptional activation, subunit 2 (150kD); CRSP complex subunit 2; DRIP150; hRGR1; human homolog of yeast RGR1; Mediator complex subunit 14; Mediator of RNA polymerase II transcription subunit 14; MGC104513; RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; thyroid hormone receptor-associated protein complex component TRAP170; transcriptional co-activator CRSP150; Transcriptional coactivator CRSP150; Trap170; vitamin D receptor-interacting protein complex component DRIP150; Vitamin D3 receptor-interacting protein complex 150 kDa component

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基因别名: 9930001L01Rik; ARC150; AU041628; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; ENSMUSG00000073278; EXLM1; Gm641; MED14; ORF1; RGR1; TRAP170

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UniProt ID: (Human) O60244, (Mouse) A2ABV5

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Entrez Gene ID: (Human) 9282, (Mouse) 26896

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功能

RNA polymerase II transcription cofactor activity transcription cofactor activity transcription coactivator activity receptor activity protein binding ligand-dependent nuclear receptor transcription coactivator activity vitamin D receptor binding thyroid hormone receptor binding general transcription factor

参与通路

regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter stem cell population maintenance intracellular steroid hormone receptor signaling pathway androgen receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated