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MED28 Antibody (CF810136) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED28 (RC204304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MED28. Positive lysates LY410842 (100 µg) and LC410842 (20 µg) can be purchased separately from OriGene.
产品信息
CF810136
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI6A3
抗原
Full length human recombinant protein of human MED28 produced in E.coli.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: endothelial-derived; endothelial-derived gene 1; Endothelial-derived protein 1; Magicin; Mediator complex subunit 28; Mediator of RNA polymerase II transcription subunit 28; mediator of RNA polymerase II transcription, subunit 28 homolog; Merlin and Grb2-interacting cytoskeletal protein; Tumor angiogenesis marker EG-1
基因别名: 1500003D12Rik; AI451633; AU045690; EG1; FKSG20; magicin; MED28; RGD1305875
UniProt ID: (Human) Q9H204, (Rat) P68943, (Mouse) Q920D3
Entrez Gene ID: (Human) 80306, (Rat) 305391, (Mouse) 66999
