Invitrogen

MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45)

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抗体检测数据 (4)

MEF2C Antibody in Immunocytochemistry (ICC/IF)

MEF2C Antibody in Immunocytochemistry (ICC/IF)

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MEF2C Antibody (MA5-46617) in ICC/IF

Immunofluorescence analysis of MEF2C was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45) (Product # MA5-46617) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More

MEF2C Antibody in Immunocytochemistry (ICC/IF)

MEF2C Antibody in Western Blot (WB)

MEF2C Antibody in Western Blot (WB)

MEF2C Antibody in Western Blot (WB)

Product Image Gallery

MEF2C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of MEF2C was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45) (Product # MA5-46617) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MEF2C Antibody in Western Blot (WB) — Western blot was performed using Anti-MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45) (Product # MA5-46617) and a 52 kDa band corresponding to MEF2C was observed across cell lines tested along with uncharacterized bands at 35 kDa. Fresh whole cell extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), TF-1 (Lane 3), Jurkat (Lane 4), U-937 (Lane 5), KARPAS 299 (Lane 6) and K-562 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). Expression of MEF2C was observed to be high in Raji, Ramos and TF-1 in comparison to Jurkat and U-937.

MEF2C Antibody in Western Blot (WB) — Western blot was performed using Anti-MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45) (Product # MA5-46617) and a 55 kDa band corresponding to MEF2C was observed across cell lines tested. Whole cell extracts (20 µg lysate) of Daudi (Lane 1), Raji (Lane2) and Jurkat (Lane 3) were electrophoresed using 4-20% SDS-PAGE gel. Resolved proteins were transferred onto a PVDF membrane. The blot was blocked with 5% BSA for 1 hour at room temperature, then probed with the primary antibody (1:500 dilution) for 2 hours at room temperature and detected by chemiluminescence with HRP labeled Goat anti-Rabbit IgG secondary antibody.

MEF2C Antibody in Western Blot (WB) — Knockdown of MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45) (Product # MA5-46617) was achieved by transfecting Raji with MEF2C specific siRNAs (Silencer® select Product # s533102 and s533103). Western blot analysis (Fig. a) was performed using fresh whole cell extracts from untransfected cells (lane 1), non-targeting scrambled siRNA transfected cells (lane 2) and MEF2C knockdown cells (lane 3). The blot was probed with MEF2C Recombinant Rabbit Monoclonal Antibody (JE35-45) (Product # MA5-46617,1:2000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MEF2C.

产品信息

MA5-46617

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE35-45

抗原

Synthetic peptide within human MEF2C aa 400-473.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2937689

产品详细信息

Positive Control: Daudi cell lysate, Raji cell lysate, Jurkat cell lysate.

Subcellular Location: Cytoplasm, Nucleus.

Tissue Specificity: Expressed in brain and skeletal muscle.Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease.

Sequence Similarities: 98% Mouse/Rat.

靶标信息

MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture. Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: MADS box transcription enhancer factor 2, polypeptide C; Myocyte enhancer factor 2C; Myocyte-specific enhancer factor 2C

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基因别名: C5DELq14.3; DEL5q14.3; MEF2C

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UniProt ID: (Human) Q06413

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Entrez Gene ID: (Human) 4208

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding AT DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding activating transcription factor binding miRNA binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity HMG box domain binding

参与通路

negative regulation of transcription from RNA polymerase II promoter MAPK cascade blood vessel development osteoblast differentiation neuron migration B cell homeostasis heart looping endochondral ossification blood vessel remodeling chondrocyte differentiation germinal center formation regulation of germinal center formation primary heart field specification secondary heart field specification outflow tract morphogenesis sinoatrial valve morphogenesis cardiac ventricle formation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process humoral immune response nervous system development heart development muscle organ development skeletal muscle tissue development muscle cell fate determination learning or memory response to virus positive regulation of gene expression negative regulation of gene expression positive regulation of alkaline phosphatase activity neural crest cell differentiation cardiac muscle hypertrophy in response to stress myotube differentiation neuron differentiation platelet formation monocyte differentiation negative regulation of ossification melanocyte differentiation positive regulation of bone mineralization positive regulation of B cell proliferation cellular response to drug skeletal muscle cell differentiation cellular response to trichostatin A B cell proliferation regulation of neuron apoptotic process negative regulation of neuron apoptotic process regulation of megakaryocyte differentiation positive regulation of myoblast differentiation positive regulation of neuron differentiation positive regulation of osteoblast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neurotransmitter secretion regulation of synaptic plasticity positive regulation of skeletal muscle tissue development neuron development cell morphogenesis involved in neuron differentiation embryonic viscerocranium morphogenesis negative regulation of epithelial cell proliferation B cell receptor signaling pathway smooth muscle cell differentiation positive regulation of muscle cell differentiation regulation of synapse assembly regulation of synaptic transmission, glutamatergic ventricular cardiac muscle cell differentiation palate development regulation of synaptic activity positive regulation of cardiac muscle cell proliferation excitatory postsynaptic potential regulation of sarcomere organization cartilage morphogenesis regulation of dendritic spine development renal tubule morphogenesis cellular response to lipopolysaccharide cellular response to calcium ion cellular response to parathyroid hormone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of cell proliferation in bone marrow glomerulus morphogenesis nephron tubule epithelial cell differentiation positive regulation of protein homodimerization activity positive regulation of macrophage apoptotic process regulation of N-methyl-D-aspartate selective glutamate receptor activity regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity positive regulation of cardiac muscle cell differentiation positive regulation of behavioral fear response epithelial cell proliferation involved in renal tubule morphogenesis positive regulation of skeletal muscle cell differentiation