Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 2
MICA Antibody (MA5-42694) in WB
Western blot analysis of MICA in extracts of various cell lines. Samples were incubated with MICA Monoclonal antibody (Product # MA5-42694) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
MA5-42694
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5A1P3
抗原
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MICA (Q29983).
偶联物
形式
Liquid
浓度
0.67 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2911835
产品详细信息
Positive test controls include: MCF7, Jurkat, OVCAR3, Mouse stomach, Rat stomach. The target is usually found in the following locations: Cell membrane, Cytoplasm, Single-pass type I membrane protein.
Immunogen sequence: HLDGQPFLRC DRQKCRAKPQ GQWAEDVLGN KTWDRETRDL TGNGKDLRMT LAHIKDQKEG LHSLQEIRVC EIHEDNSTRS SQHFYYDGEL FLSQNLETKE W
靶标信息
Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: DAMA-345G11.2; HLA class I antigen; MHC class I chain-related protein A; MHC class I polypeptide-related sequence A; MHC class I related sequence A; MIC-A; MICA; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A
基因别名: MIC-A; MICA; PERB11.1
UniProt ID: (Human) Q9TQA4
Entrez Gene ID: (Human) 100507436
T cell mediated cytotoxicity
immune response to tumor cell
cellular response to DNA damage stimulus
response to heat
cytolysis
antigen processing and presentation
negative regulation of natural killer cell activation
natural killer cell mediated cytotoxicity
defense response to bacterium
negative regulation of natural killer cell mediated cytotoxicity
gamma-delta T cell activation
regulation of immune response
defense response to virus
