Invitrogen

MICAL1 Polyclonal Antibody

查看其他10个MICAL1抗体

抗体检测数据 (4)

MICAL1 Antibody in Immunocytochemistry (ICC/IF)

MICAL1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

MICAL1 Antibody (PA5-106316) in ICC/IF

Immunofluorescent analysis of MICAL1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MICAL1 polyclonal antibody (Product # PA5-106316) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

MICAL1 Antibody in Immunocytochemistry (ICC/IF)

MICAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MICAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MICAL1 Antibody in Western Blot (WB)

Product Image Gallery

MICAL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MICAL1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MICAL1 polyclonal antibody (Product # PA5-106316) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

MICAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MICAL1 in human normal tissues adjacent to pancreatic cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MICAL1 polyclonal antibody (Product # PA5-106316) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MICAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MICAL1 in human normal tissues adjacent to mammary cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MICAL1 polyclonal antibody (Product # PA5-106316) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MICAL1 Antibody in Western Blot (WB) — Western blot analysis of MICAL1 in Jurkat cell lysate (left lane: treated with blocking peptide). Samples were incubated with MICAL1 polyclonal antibody (Product # PA5-106316).

产品信息

PA5-106316

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MICAL1(Accession Q8TDZ2), corresponding to amino acid residues E437-A487.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2853993

产品详细信息

Antibody detects endogenous levels of total MICAL1.

靶标信息

May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: [F-actin]-monooxygenase MICAL1; CasL interacting molecule MICAL; MICAL-1; microtubule associated monoxygenase, calponin and LIM domain containing 1; mMical1; Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; protein-methionine sulfoxide oxidase MICAL1

查看更多收起

基因别名: MICAL; MICAL-1; MICAL1; NICAL

查看更多收起

UniProt ID: (Human) Q8TDZ2, (Mouse) Q8VDP3

查看更多收起

Entrez Gene ID: (Human) 64780, (Mouse) 171580

查看更多收起

actin binding protein binding zinc ion binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen SH3 domain binding Rab GTPase binding FAD binding monooxygenase activity 3-(3-hydroxyphenyl)propionate hydroxylase activity oxidoreductase activity 4-chlorobenzaldehyde oxidase activity 3,5-xylenol methylhydroxylase activity phenylacetate hydroxylase activity 4-nitrophenol 4-monooxygenase activity dimethyl sulfide monooxygenase activity alpha-pinene monooxygenase [NADH] activity phenanthrene 9,10-monooxygenase activity 1-hydroxy-2-naphthoate hydroxylase activity toluene 4-monooxygenase activity xylene monooxygenase activity dibenzothiophene monooxygenase activity 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity chlorophenol 4-monooxygenase activity carbon disulfide oxygenase activity toluene 2-monooxygenase activity 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity phenanthrene 1,2-monooxygenase activity tetrahydrofuran hydroxylase activity styrene monooxygenase activity toluene-4-sulfonate monooxygenase activity toluene-sulfonate methyl-monooxygenase activity 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity 2-hydroxy-phenylacetate hydroxylase activity 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity phenanthrene 3,4-monooxygenase activity toluene 3-monooxygenase activity 4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (3-hydroxylating) activity limonene monooxygenase activity 2-methylnaphthalene hydroxylase activity 1-methylnaphthalene hydroxylase activity bisphenol A hydroxylase A activity salicylate 5-hydroxylase activity isobutylamine N-hydroxylase activity branched-chain dodecylbenzene sulfonate monooxygenase activity 3-HSA hydroxylase activity 4-hydroxypyridine-3-hydroxylase activity 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase activity 6-hydroxynicotinate 3-monooxygenase activity metal ion binding tocotrienol omega-hydroxylase activity thalianol hydroxylase activity actin or actin-binding cytoskeletal protein

参与通路

negative regulation of protein phosphorylation cytoskeleton organization signal transduction sulfur oxidation actin filament depolymerization negative regulation of cysteine-type endopeptidase activity involved in apoptotic process oxidation-reduction process negative regulation of apoptotic process