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MIF Antibody (PA5-95222) in ICC/IF
Immunofluorescence analysis of Macrophage migration inhibitory factor was performed using 70% confluent log phase U-87 MG cells treated with VEGF (100 ng/mL for 48hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MIF Polyclonal Antibody (Product # PA5-95222) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cr... View More
产品信息
PA5-95222
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human MIF recombinant protein (Position: P2-A115).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2807026
产品详细信息
Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GIF; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase
基因别名: GIF; GLIF; MIF; MMIF
UniProt ID: (Human) P14174
Entrez Gene ID: (Human) 4282
prostaglandin biosynthetic process
negative regulation of mature B cell apoptotic process
inflammatory response
cell surface receptor signaling pathway
cell aging
cell proliferation
negative regulation of gene expression
positive regulation of protein kinase A signaling
carboxylic acid metabolic process
DNA damage response, signal transduction by p53 class mediator
positive regulation of B cell proliferation
positive regulation of lipopolysaccharide-mediated signaling pathway
negative regulation of cellular protein metabolic process
negative regulation of myeloid cell apoptotic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of phosphorylation
regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of MAP kinase activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
innate immune response
positive regulation of fibroblast proliferation
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive chemotaxis
positive regulation of prostaglandin secretion involved in immune response
positive regulation of myeloid leukocyte cytokine production involved in immune response
protein homotrimerization
positive regulation of ERK1 and ERK2 cascade
negative regulation of cell cycle arrest
positive regulation of arachidonic acid secretion
negative regulation of cell aging
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
