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MLKL Antibody (PA5-115578) in ICC/IF
Immunocytochemistry analysis of MLKL in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115578). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
产品信息
PA5-115578
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from mouse MLKL(Accession Q9D2Y4), corresponding to amino acid residues A335-A355.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900213
产品详细信息
Antibody detects endogenous levels of total MLKL.
靶标信息
The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hMLKL; mixed lineage kinase domain-like; Mixed lineage kinase domain-like protein; MLKL; rCG51567-like
基因别名: 9130019I15Rik; hMLKL; MLKL
UniProt ID: (Human) Q8NB16, (Mouse) Q9D2Y4
Entrez Gene ID: (Human) 197259, (Rat) 690743, (Mouse) 74568
