Invitrogen

MLKL Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6个已发表图片

5 篇参考文献

查看其他27个MLKL抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 11

MLKL Antibody (PA5-34733) in WB

Western blot was performed using MLKL Polyclonal Antibody (Product # PA5-34733) and a 54kDa band corresponding to MLKL was observed across cell lines tested. Membrane enriched cell extracts (30 µg lysate) of HT-29 (Lane 1), THP-1 (Lane 2), U-937 (Lane 3) and HeLa (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:20... View More

Product Image Gallery

MLKL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MLKL siRNA and decrease in signal intensity was observed in western blot application using Anti-MLKL Polyclonal Antibody (Product # PA5-34733). {KD}

产品信息

PA5-34733

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看5篇已发表文章 5篇已发表文章

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contains a sequence corresponding to a region within amino acids 1 and 226 (Q8NB16) of MLKL

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.52 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2552085

产品详细信息

Recommended positive controls: THP-1, Jurkat, HCT116, PC-3.

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The gp130/STAT3-endoplasmic reticulum stress axis regulates hepatocyte necroptosis in acute liver injury.

Croatian medical journal

Li X,Wang J,Li Y,He W,Cheng QJ,Liu X,Xu DL,Jiang ZG,Xiao X,He YH

Published figure using MLKL polyclonal antibody (Product # PA5-34733) in Western Blot

Fri Jun 30 00:00:00 EDT 2023

Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis.

BMC microbiology

Jeon YJ,Gil CH,Won J,Jo A,Kim HJ

Published figure using MLKL polyclonal antibody (Product # PA5-34733) in Western Blot

Fri May 26 00:00:00 EDT 2023

Intracellular alpha-fetoprotein mitigates hepatocyte apoptosis and necroptosis by inhibiting endoplasmic reticulum stress.

World journal of gastroenterology

Chen YF,Liu SY,Cheng QJ,Wang YJ,Chen S,Zhou YY,Liu X,Jiang ZG,Zhong WW,He YH

PA5-34733 was used in Western Blotting to investigate whether and how AFP could regulate ER stress and hepatocyte injury.

Thu Jul 14 00:00:00 EDT 2022

Tumor necrosis factor is a necroptosis-associated alarmin.

Frontiers in immunology

Pinci F,Gaidt MM,Jung C,Nagl D,Kuut G,Hornung V

PA5-34733 was used in Immunoprecipitation, Western Blot to demonstrate that lytic cell death, particularly necroptosis, is a critical trigger for TNF release and thus qualify TNF as a necroptosis-associated alarmin.

Fri Dec 30 00:00:00 EST 2022

Downregulation of RIP3 Improves the Protective Effect of ATF6 in an Acute Liver Injury Model.

BioMed research international

Huang MY,Wan DW,Deng J,Guo WJ,Huang Y,Chen H,Xu DL,Jiang ZG,Xue Y,He YH

PA5-34733 was used in Western Blotting to investigate the impact of Activating transcription factor 6 on receptor-interacting protein 3 expression, and its role in hepatocyte necroptosis in an acute liver injury model.