Invitrogen

MPP8 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他9个MPP8抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (5)
高级验证 (1)

MPP8 Antibody in Immunohistochemistry (IHC)

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MPP8 Antibody (PA5-59036) in IHC

Immunohistochemical staining of MPP8 in human cerebral cortex, kidney, lymph node and testis using MPP8 Polyclonal Antibody (Product # PA5-59036) (A) shows similar protein distribution across tissues to an independent MPP8 Polyclonal Antibody (B).

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MPP8 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of MPP8 demonstrates antibody specificity. Immunohistochemical analysis of MPP8 using anti-MPP8 Polyclonal Antibody (Product # PA5-59036), shows significant staining of MPP8 in human thyroid gland and shows minimal or weak staining in human liver tissues. The relative expression levels of MPP8 within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-59036

应用

建议稀释比

已发表文章

免疫组化 (IHC)

1:1,000-1:2,500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human MPP8. Recombinant protein control fragment (Product #RP-98008).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2644084

产品详细信息

Immunogen sequence: KRHDSDKEEK GRKEPKGLKT LKEIRNAFDL FKLTPEEKND VSENNRKREE IPLDFKTIDD HKTKENKQSL KERRNTRDET DTWAYIAAEG DQEVLDSVCQ ADENSDGR

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 77%.

靶标信息

Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。