Invitrogen

MSH2 Polyclonal Antibody

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MSH2 Antibody in Western Blot (WB)

MSH2 Antibody in Western Blot (WB)

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MSH2 Antibody (PA5-29160) in WB

Western Blot using MSH2 Polyclonal Antibody (Product # PA5-29160). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431 . 7.5% SDS PAGE. MSH2 Polyclonal Antibody (Product # PA5-29160) diluted at 1:1,000.

MSH2 Antibody in Western Blot (WB)

MSH2 Antibody in Western Blot (WB)

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MSH2 Antibody in Western Blot (WB) — Western Blot using MSH2 Polyclonal Antibody (Product # PA5-29160). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431 . 7.5% SDS PAGE. MSH2 Polyclonal Antibody (Product # PA5-29160) diluted at 1:1,000.

MSH2 Antibody in Western Blot (WB) — Figure 3 The expression of proteins associated with colorectal carcinoma (CRC) tumorigenesis and metastasis was determined in the novel African American CRC lines by immunoblotting. (A) Qualitative analysis of CHTN06, SB501 and SB521 and HT-29, a Caucasian American CRC cell line, for protein expression of beta-catenin, p53, nuclear factor (NF)-kappaB (p50 and p65), villin-1, MSH2, MSH6, MLH1 and ezrin. (B) Semi-quantitative densitometry was performed by normalizing protein expression to the respective beta-tubulin loading control. Data were generated from three independent experiments. CEA, carcinoembryonic antigen.

产品信息

PA5-29160

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 648 and 922 of Human MSH2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.01 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546636

产品详细信息

Recommended positive controls: 293T, A431, Raji.

Predicted reactivity: Mouse (93%), Rat (92%), Pig (93%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity.

International journal of oncology

Paredes J,Ji P,Lacomb JF,Shroyer KR,Martello LA,Williams JL

Published figure using MSH2 polyclonal antibody (Product # PA5-29160) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

生物信息学

蛋白别名: DNA mismatch repair protein Msh2; hMSH2; Msh2p; mutS homolog 2, colon cancer, nonpolyposis type 1; MutS protein homolog 2

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基因别名: COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2

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UniProt ID: (Human) P43246

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Entrez Gene ID: (Human) 4436

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magnesium ion binding four-way junction DNA binding Y-form DNA binding heteroduplex DNA loop binding double-strand/single-strand DNA junction binding DNA binding double-stranded DNA binding single-stranded DNA binding protein binding ATP binding protein C-terminus binding ATPase activity centromeric DNA binding enzyme binding protein kinase binding mismatched DNA binding guanine/thymine mispair binding dinucleotide insertion or deletion binding single guanine insertion binding single thymine insertion binding dinucleotide repeat insertion binding oxidized purine DNA binding MutLalpha complex binding protein homodimerization activity ADP binding DNA metabolism protein

参与通路

meiotic mismatch repair in utero embryonic development oxidative phosphorylation DNA repair mismatch repair postreplication repair double-strand break repair meiotic gene conversion cell cycle arrest reciprocal meiotic recombination germ cell development determination of adult lifespan male gonad development response to X-ray response to UV-B somatic hypermutation of immunoglobulin genes somatic recombination of immunoglobulin gene segments B cell mediated immunity B cell differentiation intra-S DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of neuron apoptotic process maintenance of DNA repeat elements negative regulation of reciprocal meiotic recombination isotype switching negative regulation of DNA recombination positive regulation of helicase activity