Invitrogen

MSX2 Polyclonal Antibody

查看其他10个MSX2抗体

抗体检测数据 (4)

MSX2 Antibody in Immunocytochemistry (ICC/IF)

MSX2 Antibody in Immunocytochemistry (ICC/IF)

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MSX2 Antibody (PA5-106670) in ICC/IF

Immunocytochemistry analysis of MSX2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MSX2 polyclonal antibody (Product # PA5-106670) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

MSX2 Antibody in Immunocytochemistry (ICC/IF)

MSX2 Antibody in Western Blot (WB)

MSX2 Antibody in Western Blot (WB)

MSX2 Antibody in Western Blot (WB)

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MSX2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of MSX2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MSX2 polyclonal antibody (Product # PA5-106670) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

MSX2 Antibody in Western Blot (WB) — Western blot analysis of MSX2 in COLO205 cell lysate (left lane: treated with blocking peptide). Samples were incubated with MSX2 polyclonal antibody (Product # PA5-106670).

MSX2 Antibody in Western Blot (WB) — Western blot analysis of MSX2 in COLO205 cells. Samples were incubated with MSX2 polyclonal antibody (Product # PA5-106670).

MSX2 Antibody in Western Blot (WB) — Western blot analysis of MSX2 in HepG2. Samples were incubated with MSX2 polyclonal antibody (Product # PA5-106670).

产品信息

PA5-106670

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MSX2(Accession P35548), corresponding to amino acid residues L79-M129.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2854338

产品详细信息

Antibody detects endogenous levels of total Msx2/Hox8.

靶标信息

Msx2 is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. This protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in the Msx2 gene are associated with parietal foramina 1 and craniosynostosis type 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: homeo box, msh-like 2; Homeobox protein Hox-8; Homeobox protein Hox-8-1; Homeobox protein MSX-2; homeobox, msh-like 2; msh homeo box 2; msh homeobox homolog 2

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基因别名: BB122635; CRS2; FPP; Hox-8; Hox-8.1; HOX8; Hox8.1; MSH; Msx-2; MSX2; PFM; PFM1

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UniProt ID: (Human) P35548, (Mouse) Q03358

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Entrez Gene ID: (Human) 4488, (Mouse) 17702

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription cofactor activity protein binding transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, transcription factor binding DNA binding homeodomain transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation chondrocyte development osteoblast development outflow tract septum morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation endochondral bone growth transcription from RNA polymerase II promoter negative regulation of cell proliferation anterior/posterior pattern specification signal transduction involved in regulation of gene expression positive regulation of BMP signaling pathway negative regulation of CREB transcription factor activity embryonic forelimb morphogenesis embryonic hindlimb morphogenesis wound healing, spreading of epidermal cells embryonic nail plate morphogenesis negative regulation of apoptotic process negative regulation of fat cell differentiation negative regulation of keratinocyte differentiation positive regulation of osteoblast differentiation negative regulation of transcription, DNA-templated stem cell differentiation positive regulation of catagen bone trabecula formation cranial suture morphogenesis frontal suture morphogenesis branching involved in mammary gland duct morphogenesis BMP signaling pathway involved in heart development enamel mineralization cellular response to estradiol stimulus activation of meiosis negative regulation of transcription regulatory region DNA binding positive regulation of mesenchymal cell apoptotic process ossification outflow tract morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development embryonic limb morphogenesis BMP signaling pathway wound healing odontogenesis anagen embryonic digit morphogenesis regulation of apoptotic process positive regulation of apoptotic process cartilage development bone morphogenesis mammary gland epithelium development cellular response to growth factor stimulus