Invitrogen

MT-ATP6 Polyclonal Antibody

MT-ATP6 Antibody in Immunocytochemistry (ICC/IF)

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MT-ATP6 Antibody (PA5-103625) in ICC/IF

Immunofluorescent analysis of MT-ATP6 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MT-ATP6 polyclonal antibody (Product # PA5-103625) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

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产品信息

PA5-103625

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MT-ATP6(Accession P00846), corresponding to amino acid residues S34-L84.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2815990

产品详细信息

Antibody detects endogenous levels of total MT-ATP6.

靶标信息

The MT-ATP6 gene provides information for making a protein that is essential for normal mitochondrial function. Mitochondria are structures within cells that convert the energy from food into a form that cells can use. These cellular structures produce energy through a process called oxidative phosphorylation, which uses oxygen and simple sugars to create adenosine triphosphate (ATP), the cell's main energy source. The MT-ATP6 protein forms one part (subunit) of a large enzyme called ATP synthase. This enzyme, which is also known as complex V, is responsible for the final step of oxidative phosphorylation. Specifically, one segment of ATP synthase allows positively charged particles, called protons, to flow across a specialized membrane inside mitochondria. Another segment of the enzyme uses the energy created by this proton flow to convert a molecule called adenosine diphosphate (ADP) to ATP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。