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MT-CYB Antibody (PA5-100740) in ICC/IF
Immunofluorescent analysis of MT-CYB in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MT-CYB polyclonal antibody (Product # PA5-100740) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100740
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MT-CYB(Accession P00156), corresponding to amino acid residues M1-T47.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850243
产品详细信息
Antibody detects endogenous levels of total MT-CYB.
靶标信息
CYTB belongs to the cytochrome b family. It is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Complex III subunit 3; Complex III subunit III; Cytochrome b; Cytochrome b-c1 complex subunit 3; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit; UQCR3
基因别名: COB; CYTB; MT-CYB; mt-Cytb; MTCYB
UniProt ID: (Mouse) P00158, (Human) P00156, (Rat) P00159
Entrez Gene ID: (Mouse) 17711, (Human) 4519, (Rat) 26192
