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MTH1 Antibody (PA5-52963) in WB
Western blot analysis of MTH1 in control (vector only transfected HEK293T lysate) and NUDT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MTH1 Polyclonal Antibody (Product # PA5-52963).
产品信息
PA5-52963
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein corresponding to Human MTH1. Recombinant protein control fragment (Product #RP-89472).
偶联物
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 40% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2644260
产品详细信息
Immunogen sequence: RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL
Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 83%.
靶标信息
Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Among the various classes of DNA damage caused by oxygen radicals, an oxidized form of guanine base (8-oxoguanine) appears to be important as it can pair with cytosine and adenine and G:C to T:A transversion mutation occurs. A significant amount of 8-OxoG is formed in the chromosomal DNA of mammalian cells, with most damaged nucleotides excised from the DNA and excreted in the urine. Along with 8-oxoG being present in oxidatively damaged DNA, 8-oxo-deoxyguanosine (8-oxo-dGTP) is formed in the nucleotide pool during normal cellular metabolism and following oxidative stress. The 8-oxo-dGTP nucleotide can be incorporated in DNA during polymerization and can result in a mispairing unless repaired. MTH converts 8-oxo-dGTP in the nucleotide pool to the monophosphate and prevents the misincorporation of 8-oxo-dGTP into DNA (Figure courtesy of Dr. Mark Kelley). MTH also recognizes 8-oxo-rGTP, which could incorporate into RNA during gene transcription leading to missense or nonsense protein production.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 2-hydroxy-dATP diphosphatase; 7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; Methylated purine nucleoside triphosphate hydrolase; mutT human homolog 1; Nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1; nudix (nucleoside diphosphate linked moiety X)-type motif 1; Nudix motif 1; Oxidized purine nucleoside triphosphate hydrolase
基因别名: MTH1; NUDT1
UniProt ID: (Human) P36639
Entrez Gene ID: (Human) 4521
GTPase activity
protein binding
8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity
snoRNA binding
8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity
GTP diphosphatase activity
2-hydroxy-adenosine triphosphate pyrophosphatase activity
2-hydroxy-(deoxy)adenosine-triphosphate pyrophosphatase activity
metal ion binding
ATP diphosphatase activity
m7G(5')pppN diphosphatase activity
hydrolase
phosphatase
metabolite interconversion enzyme
