Invitrogen

MUC4 Monoclonal Antibody (1G8)

MUC4 Antibody in Immunocytochemistry (ICC/IF)

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MUC4 Antibody (35-4900) in ICC/IF

Immunofluorescence analysis of MUC4/Mucin 4 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC4/Mucin 4 (1G8) Mouse Monoclonal Antibody (Product # 35-4900) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

MUC4 Antibody in Immunohistochemistry (IHC)

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产品信息

35-4900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

Assay-dependent

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免疫细胞化学 (ICC/IF)

2 µg/mL

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Bovine, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1G8

抗原

Rat ASGP-2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533206

靶标信息

Nearly all mucosal surfaces are protected by mucin transmembrane glycoproteins, from bronchioles to corneas to reproductive tracts. Mucin 4 (MUC4) is membrane-bound and is important in protecting and lubricating. MUC4 expression is erroneous in many carcinomas and sarcomas, including fibromyoxoid sarcoma and pancreatic adenocarcinoma. Immunohistochemical staining for MUC4 has been discussed as a potential biomarker for detection of these cancers. MUC4 seems to be most predominantly affected in Asian diseases - Taiwanese infertility and endometriosis, as well as gallstone formation in Chinese men, are connected to MUC4, where similar diseases in other populations do not share these MUC4 mutations. Because MUC4 is so important in mucosal surface protection, it is not surprising that it has been implicated as failing in many metastatic cancer invasions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transcorneal but not transpalpebral electrical stimulation disrupts mucin homeostasis of the ocular surface.

BMC ophthalmology

Yang M,Lennikov A,Chang K,Ashok A,Lee C,Cho KS,Utheim TP,Dartt DA,Chen DF

35-4900 was used in Western Blotting to conclude that TcES, but not TpES, induces corneal epithelial damage in mice by disrupting mucin homeostasis, thus TpES may represent a safer and more effective ES approach for treating retinal neurodegeneration clinically.

Thu Dec 15 00:00:00 EST 2022

Transcorneal but not transpalpebral electrical stimulation disrupts mucin homeostasis of the ocular surface.

BMC ophthalmology

Yang M,Lennikov A,Chang K,Ashok A,Lee C,Cho KS,Utheim TP,Dartt DA,Chen DF

Thu Dec 15 00:00:00 EST 2022

Isolation and characterization of adult mammary stem cells from breast cancer-adjacent tissues.

Oncology letters

Shi AP,Fan ZM,Ma KW,Jiang YF,Wang L,Zhang KW,Fu SB,Xu N,Zhang ZR

35-4900 was used in Immunocytochemistry to compare the differentiation abilities of normal adult mammary stem cells with autologous primary breast cancer cells.

Fri Sep 01 00:00:00 EDT 2017

Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats.

Scientific reports

Leng Y,Yi M,Fan J,Bai Y,Ge Q,Yao G

35-4900 was used in immunohistochemistry to study intra-abdominal hypertension-induced changes in gastrointestinal permeability

Wed Mar 16 00:00:00 EDT 2016

Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.

Nature structural & molecular biology

Dhir A,Dhir S,Proudfoot NJ,Jopling CL

35-4900 was used in immunohistochemistry - paraffin section to study transcription of lncRNA genes that also encode for miRNAs

Wed Apr 01 00:00:00 EDT 2015

Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.

Diagnostic pathology

Ertoy Baydar D,Kosemehmetoglu K,Aydin O,Bridge JA,Buyukeren B,Aki FT

35-4900 was used in immunohistochemistry to present two cases of primary sclerosing epithelioid fibrosarcoma of the kidney

Fri Oct 09 00:00:00 EDT 2015

Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.

Diagnostic pathology

Ertoy Baydar D,Kosemehmetoglu K,Aydin O,Bridge JA,Buyukeren B,Aki FT

35-4900 was used in immunohistochemistry to present two cases of primary sclerosing epithelioid fibrosarcoma of the kidney

Fri Oct 09 00:00:00 EDT 2015

Tau and PTEN status as predictive markers for response to trastuzumab and paclitaxel in patients with HER2-positive breast cancer.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Koo DH,Lee HJ,Ahn JH,Yoon DH,Kim SB,Gong G,Son BH,Ahn SH,Jung KH

35-4900 was used in immunohistochemistry - paraffin section to identify biomarkers using HER2-positive advanced breast cancer samples from patients treated with trastuzumab and paclitaxel combination chemotherapy

Sat Aug 01 00:00:00 EDT 2015

Universal antibody conjugation to nanoparticles using the Fcγ receptor I (FcγRI): quantitative profiling of membrane biomarkers.

Bioconjugate chemistry

Kim C,Galloway JF,Lee KH,Searson PC

35-4900 was used in immunocytochemistry to develop a method for universal conjugation of antibodies to lipid-coated nanoparticles using radially oriented FcgammaRIs

Wed Oct 15 00:00:00 EDT 2014

Pancreatic cancer counterattack: MUC4 mediates Fas-independent apoptosis of antigen-specific cytotoxic T lymphocyte.

Oncology reports

Zhu Y,Zhang JJ,Liang WB,Zhu R,Wang B,Miao Y,Xu ZK

35-4900 was used in Flow cytometry/Cell sorting to identify a possible novel MUC4-mediated pathway by which pancreatic cancer cells induce the apoptosis of antigen-specific cytotoxic T lymphocytes.

Tue Apr 01 00:00:00 EDT 2014

In situ drug-receptor binding kinetics in single cells: a quantitative label-free study of anti-tumor drug resistance.

Scientific reports

Wang W,Yin L,Gonzalez-Malerva L,Wang S,Yu X,Eaton S,Zhang S,Chen HY,LaBaer J,Tao N

35-4900 was used in Immunocytochemistry-immunoflourescence to investigate the molecular scale origin of anti-tumour drug resistance.

Tue Oct 14 00:00:00 EDT 2014

Transmembrane mucin MUC1 overexpression and its association with CD10⁺ myeloid cells, transforming growth factor-β1 expression, and tumor budding grade in colorectal cancer.

Cancer science

Khanh do T,Mekata E,Mukaisho K,Sugihara H,Shimizu T,Shiomi H,Murata S,Naka S,Yamamoto H,Endo Y,Tani T

35-4900 was used in Immunohistochemistry to show MUC1 expression appears to be a chemoattractant for CD10⁺ stromal cells.

Mon Jul 01 00:00:00 EDT 2013

Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology.

Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society

Noda Y,Fujita N,Kobayashi G,Ito K,Horaguchi J,Hashimoto S,Koshita S,Ishii S,Kanno Y,Ogawa T,Masu K,Tsuchiya T,Oikawa M,Honda H,Sawai T,Uzuki M,Fujishima F

35-4900 was used in immunohistochemistry to compare the diagnostic efficacies of cell block method and conventional smear method for bile cytology

Mon Jul 01 00:00:00 EDT 2013

Mucin 16 is a functional selectin ligand on pancreatic cancer cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chen SH,Dallas MR,Balzer EM,Konstantopoulos K

35-4900 was used in Flow Cytometry to identify mucin 16 as a novel selectin ligand expressed by metastatic pancreatic cancer cells.

Thu Mar 01 00:00:00 EST 2012

Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA.

World journal of gastroenterology

Yeh CN,Lin KJ,Chen TW,Wu RC,Tsao LC,Chen YT,Weng WH,Chen MF

35-4900 was used in immunohistochemistry to establish and characterize a thioacetamide-induced culture model of CCA rat cells.

Tue Jun 28 00:00:00 EDT 2011

Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA.

World journal of gastroenterology

Yeh CN,Lin KJ,Chen TW,Wu RC,Tsao LC,Chen YT,Weng WH,Chen MF

35-4900 was used in immunohistochemistry to establish and characterize a thioacetamide-induced culture model of CCA rat cells.

Tue Jun 28 00:00:00 EDT 2011

AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells.

Biochemical and biophysical research communications

Bae CH,Kim JW,Ye SB,Song SY,Kim YW,Park SY,Kim YD

35-4900 was used in ELISA to study the mechanism by which AMPK induces MUC5B expression in airway epithelial cells.

Fri Jun 17 00:00:00 EDT 2011

Leptin up-regulates MUC5B expression in human airway epithelial cells via mitogen-activated protein kinase pathway.

Experimental lung research

Woo HJ,Yoo WJ,Bae CH,Song SY,Kim YW,Park SY,Kim YD

35-4900 was used in ELISA to investigate the mechanism of MUC5B upregulation induced by leptin

Tue Jun 01 00:00:00 EDT 2010

Prognostic value of MUC4 for mass-forming intrahepatic cholangiocarcinoma after hepatectomy.

Oncology reports

Yeh CN,Pang ST,Wu RC,Chen TW,Jan YY,Chen MF

35-4900 was used in immunohistochemistry to assess the prognostic value of MUC4 for predicting the overall survival rate for mass-forming-cholangiocarcinoma patients undergoing hepatectomy

Thu Jan 01 00:00:00 EST 2009

Glycoproteomics of milk: differences in sugar epitopes on human and bovine milk fat globule membranes.

Journal of proteome research

Wilson NL,Robinson LJ,Donnet A,Bovetto L,Packer NH,Karlsson NG

Mon Sep 01 00:00:00 EDT 2008

Glycoproteomics of milk: differences in sugar epitopes on human and bovine milk fat globule membranes.

Journal of proteome research

Wilson NL,Robinson LJ,Donnet A,Bovetto L,Packer NH,Karlsson NG

Mon Sep 01 00:00:00 EDT 2008

Differential regulation of membrane-associated mucins in the human ocular surface epithelium.

Investigative ophthalmology & visual science

Hori Y,Spurr-Michaud S,Russo CL,Argüeso P,Gipson IK

Thu Jan 01 00:00:00 EST 2004

生物信息学

蛋白别名: Ascites sialoglycoprotein; ASGP; ASGP1; MUC-4; mucin 4, ASGP; mucin 4, tracheobronchial; Mucin-4; Mucin4; Pancreatic adenocarcinoma mucin; pre-sialomucin complex; sialomucin ascites sialoglycoprotein-1; sialomucin complex; Testis mucin; Tracheobronchial mucin

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基因别名: 4933405I11Rik; ASGP; ASGP-1; HSA276359; MUC-4; MUC4; Psmc

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UniProt ID: (Human) Q99102, (Mouse) Q8JZM8

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Entrez Gene ID: (Human) 4585, (Rat) 303887, (Mouse) 140474

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功能

ErbB-2 class receptor binding extracellular matrix constituent, lubricant activity protein complex binding

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