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Invitrogen
MUC5B Monoclonal Antibody (5B19-2E)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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MUC5B Antibody (37-7400) in ICC/IF
Immunofluorescence analysis of MUC5B was performed using 70 % confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC5B (5B19-2E) Mouse Monoclonal antibody (Product # 37-7400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
产品信息
37-7400
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
5B19-2E
抗原
Synthetic peptide derived from the N-terminal region of the human MUC5B protein.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533338
产品详细信息
Both IHC and WB applications require the following deglycosylation protocols.
Immunohistochemistry
1. Prepare the following stock solutions:
a. 200 mM NaIO4 (8.556 g/200 mL water)
b. Sodium-Acetate buffer, pH 4.5: Dissolve NaCl (final concentration: 0.33M) into 0.1M glacial acetic acid and adjust to pH 4.5 using 1 N NaOH.
c. Sodium-thiosulfate solution: Dissolve Na2S2O3 (0.133M), NaI (0.033M), and NaHCO3 (0.033M) in water and adjust to pH 7.6 using 1M HCl. Keep solution at 4°C.
2. After deparaffinization, rehydration, and peroxidase quenching steps, mix a 1:1 solution of “a” and “b” (in step 1), add to each slide, and incubate overnight at 4°C.
3. Treat tissue with solution “c” for 30 minutes at 4°C.
4. Proceed with normal immunostaining protocol (blocking and antibody incubation steps).
Western blotting
1. Treat transblotted membrane with 10 mM NaIO4 in 0.1M sodium acetate/5 mM EDTA, pH 5.5 overnight at 4°C in the dark.
2. After washing off the solution, incubate the membrane in a solution containing 30 mM Na2S2O3, 7.5 mM NaI, and 7.5 mM NaHCO3, pH 7.2 for 1 hour at 4°C in the dark.
3. Proceed with normal Western blot protocol (wash and blocking steps).
靶标信息
Mucins (MUCs) are heavily glycosylated glycoproteins that are synthesized either as membrane-bound or secreted proteins. Mucins are noteworthy because they have an aberrant expression profile in various malignancies. Mucins normally play a protective role for epithelial tissues and are expressed in the gastrointestinal, respiratory, and genito-urinary tracts. Mucins may also be involved in the renewal and differentiation of the epithelium, and the modulation of cell adhesion and cell signaling. MUC5B is thought to play a vital role in maintaining oral health, and may also be involved in respiratory and oral disease.2 MUC5B expression has been studied in gastric carcinoma as well as chronic obstructive pulmonary disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cervical mucin; cervical mucin MUC5B; High molecular weight salivary mucin MG1; MUC-5B; mucin 5, subtype B, tracheobronchial; Mucin-5 subtype B, tracheobronchial; Mucin-5B; Sublingual gland mucin
基因别名: MG1; MUC-5B; MUC5; MUC5B; MUC9
UniProt ID: (Human) Q9HC84
Entrez Gene ID: (Human) 727897
