Invitrogen

MVP Polyclonal Antibody

MVP Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

MVP Antibody (PA5-80836) in ICC/IF

Immunofluorescence staining of MVP in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with MVP Polyclonal Antibody (Product # PA5-80836, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

MVP Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

PA5-80836

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

免疫沉淀 (IP)

1-4 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the C-terminus of the Human MVP/major vault protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2788105

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human MVP/major vault protein.

靶标信息

This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.

仅用于科研。不用于诊断过程。未经明确授权不得转售。