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MYLK Antibody (MA5-35409) in WB
Western blot analysis of MYLK in extracts of various cell lines. Samples were incubated with MYLK Monoclonal antibody (Product # MA5-35409) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
产品信息
MA5-35409
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC0842
抗原
A synthetic peptide corresponding to a sequence within amino acids 1800-1900 of human MYLK (Q15746)
偶联物
形式
Liquid
浓度
0.65 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849310
产品详细信息
Immunogen sequence: VAEEKPHVKP YFSKTIRDLE VVEGSAARFD CKIEGYPDPE VVWFKDDQSI RESRHFQIDY DEDGNCSLII SDVCGDDDAK YTCKAVNSLG EATCTAELIV E
靶标信息
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Kinase-related protein; KRP; MLCK; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin
基因别名: AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK1; smMLCK
UniProt ID: (Human) Q15746
Entrez Gene ID: (Human) 4638, (Rat) 288057
protein phosphorylation
smooth muscle contraction
actin filament organization
tonic smooth muscle contraction
positive regulation of cell migration
bleb assembly
positive regulation of calcium ion transport
aorta smooth muscle tissue morphogenesis
cellular hypotonic response
positive regulation of wound healing
positive regulation of defense response to virus by host
muscle contraction
mitophagy in response to mitochondrial depolarization
xenophagy
