Bethyl Laboratories

Menin Polyclonal Antibody

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抗体检测数据 (4)

Menin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Menin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Menin Antibody (A300-105A) in IHC (P)

Detection of human Menin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Menin (Product # A300-105A) (Lot 13) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Menin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Menin Antibody in Western Blot (WB)

Menin Antibody in Western Blot (WB)

Menin Antibody in Immunoprecipitation (IP)

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Menin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human Menin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Menin (Product # A300-105A) (Lot 13) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Menin Antibody in Western Blot (WB) — Detection of mouse Menin by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody (Product # A300-105A lot 13) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Menin Antibody in Western Blot (WB) — Detection of human Menin by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody (Product # A300-105A lot 13) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Menin Antibody in Immunoprecipitation (IP) — Detection of human Menin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (Product # A300-105A) (lot 13) used for IP at 6 µg per reaction. Menin was also immunoprecipitated by a previous lot of this antibody (Product # A300-105A) (lot 12) and a second antibody against a different epitope of Menin (Product # A300-115A). For blotting immunoprecipitated Menin, A300-105A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

产品信息

A300-105A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000-1:25,000

-

免疫组化（石蜡） (IHC (P))

1:500-1:2,000

-

免疫沉淀 (IP)

2-10 µg/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Dog

靶标信息

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Menin; multiple endocrine neoplasia I

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基因别名: AW045611; MEAI; MEN1; SCG2

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UniProt ID: (Human) O00255, (Mouse) O88559

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Entrez Gene ID: (Human) 4221, (Mouse) 17283

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four-way junction DNA binding Y-form DNA binding chromatin binding double-stranded DNA binding protein binding histone-lysine N-methyltransferase activity protein binding, bridging transcription regulatory region DNA binding protein N-terminus binding R-SMAD binding DNA binding sequence-specific DNA binding

参与通路

negative regulation of transcription from RNA polymerase II promoter MAPK cascade ossification leukocyte homeostasis negative regulation of protein phosphorylation osteoblast fate commitment osteoblast development type B pancreatic cell differentiation chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus cell cycle arrest negative regulation of cell proliferation response to UV embryo development response to gamma radiation negative regulation of cell-substrate adhesion chromatin modification histone methylation hemopoiesis positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone methylation positive regulation of protein binding regulation of activin receptor signaling pathway histone lysine methylation positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of cell differentiation negative regulation of osteoblast differentiation positive regulation of osteoblast differentiation negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of JNK cascade negative regulation of organ growth embryonic skeletal system morphogenesis negative regulation of epithelial cell proliferation positive regulation of cell division negative regulation of telomerase activity palate development maternal process involved in female pregnancy regulation of type B pancreatic cell proliferation response to transforming growth factor beta negative regulation of cell cycle G1/S phase transition mitotic cell cycle DNA repair brain development decidualization cellular response to glucose stimulus cellular response to peptide hormone stimulus beta-catenin-TCF complex assembly