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Midkine Antibody (500-P171-1MG) in WB
Western Blot: To detect Human Midkine by Western Blot analysis Midkine Polyclonal Antibody (Product # 500-P171-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Midkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P171-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human Midkine
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929617
产品详细信息
AA Sequence of recombinant protein: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine. Anti-Human Midkine-specific antibody was purified by affinity chromatography employing an immobilized Human Midkine matrix.
Sandwich ELISA: To detect Human Midkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Midkine (500-P171Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Midkine.
Western Blot: To detect Human Midkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Midkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Amphiregulin-associated protein; ARAP; FLJ27379; Midgestation and kidney protein; Midkine; MK; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; retinoic acid inducible factor
基因别名: ARAP; MDK; MK; MK1; NEGF2
UniProt ID: (Human) P21741
Entrez Gene ID: (Human) 4192
behavioral fear response
signal transduction
Notch signaling pathway
nervous system development
short-term memory
response to wounding
cell migration
dentate gyrus development
cerebellar granular layer development
cerebral cortex development
cell differentiation
adrenal gland development
defecation
response to drug
negative regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
regulation of behavior
response to glucocorticoid
positive regulation of cell division
