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Invitrogen
Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™
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Mouse IgG2a kappa Isotype Control (11-4724-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD11c APC (Product # 17-0116-42), Anti-Human CD370 (Clec9A) PE (Product # 12-3709-42), and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD141 FITC (right). CD11c-positive cells were used for analysis.
产品信息
11-4724-42
应用
建议稀释比
免疫组化 (IHC)
Assay-Dependent
免疫细胞化学 (ICC/IF)
Assay-Dependent
流式细胞分析 (Flow)
5 µL (1 µg)/test
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBM2a
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1963642
产品详细信息
Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported:This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis, immunocytochemistry, and immunohistochemistry.
Applications Tested: Has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL 1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
