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Invitrogen
Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 645, eBioscience™
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Mouse IgG2b kappa Isotype Control (64-4732-82)
Normal human peripheral blood cells were unstained (left), stained with Mouse IgG2b kappa Isotype Control Monoclonal Antibody, Super Bright 645 (middle), or stained with CD45RO Monoclonal Antibody, Super Bright 645 (Product # 64-0457-42) (right). Cells in the lymphocyte gate were used for analysis.
产品信息
64-4732-82
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBMG2b
偶联物
激发/发射光谱
414/645 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2724721
产品详细信息
Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.
Applications Reported: Mouse IgG2b Isotype Control Super Bright 645 has been reported for use in flow cytometric analysis.
Applications Tested: Mouse IgG2b Isotype Control Super Bright 645 has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.
Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
靶标信息
Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
