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Invitrogen
Mouse anti-Human IgM (Heavy chain) Secondary Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Human IgM (Heavy chain) Secondary Antibody (05-4900) in ICC/IF
Immunofluorescence analysis of Mouse anti-Human IgM (Heavy chain) Secondary Antibody was performed using log phase Ramos cells (IgM producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with0.1% Triton™ X-100 for 15minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Mouse anti-Human IgM (Heavy chain) Secondary Antibody (P... View More
产品信息
05-4900
应用
建议稀释比
免疫印迹 (WB)
1:500-1:20,000
免疫组化（石蜡） (IHC (P))
1:500-1:20,000
免疫细胞化学 (ICC/IF)
1:250
酶联免疫吸附实验 (ELISA)
1:500-1:20,000
免疫沉淀 (IP)
1:500-1:20,000
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
HP6083
抗原
Human IgM
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2532927
靶标
IgM (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
This antibody does not react with IgA, IgG or light chains.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
