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Human IgM Secondary Antibody (MA5-14729) in WB
Western blot analysis of IgM was performed by loading 1 µg of purified Human IgM, Human IgA, and Human IgG and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgM heavy chain was detected using a mouse anti-human IgM monoclonal antibody (Product # MA5-14729) diluted to a concentration of 2 µg/mL in blocking buffer,... View More
产品信息
MA5-14729
应用
建议稀释比
免疫印迹 (WB)
1:100-1:400
流式细胞分析 (Flow)
1 µg/mL
酶联免疫吸附实验 (ELISA)
1 µg/mL
免疫沉淀 (IP)
Assay-dependent
放射免疫测定 (RIA)
Assay-dependent
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
P5E2
抗原
Purified Human Serum IgM
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
PBS, pH 7.4, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10981883
靶标
IgM
抗体形式
Whole Antibody
产品详细信息
By ELISA and WB, MA5-14712 does not react with Human IgG or Human IgA.
Product MA514729 is a smaller package size of MII0401 (formerly sold as a Seradyn product).
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
