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Invitrogen
Mouse anti-Rat IgG2a Secondary Antibody, FITC, eBioscience™
产品信息
11-4817-82
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
Assay-Dependent
免疫组化（冰冻） (IHC (F))
Assay-dependent
免疫细胞化学 (ICC/IF)
Assay-Dependent
流式细胞分析 (Flow)
0.5 µg/test
产品规格
种属反应
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Secondary Antibody
克隆号
r2a-21B2
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11042292
靶标
IgG2a
抗体形式
Whole Antibody
产品详细信息
Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.
Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis and immunohistochemstry using rat IgG2a primary antibody. This can be used at less than or equal to 0.5 µg per test for flow cytometry or 10 µg/mL for IHC. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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