Product References
High-valency Anti-CD99 Antibodies Toward the Treatment of T Cell Acute Lymphoblastic Leukemia.
Journal of molecular biology
Romero LA,Hattori T,Ali MAE,Ketavarapu G,Koide A,Park CY,Koide S
A-21281 was used in Flow Cytometry to develop a human IgG-based tetravalent version of 10A1 that exhibited cytotoxic activity to T-ALL cells but not to healthy peripheral blood cells.
Tue Mar 15 00:00:00 EDT 2022
Generation of recombinant hyperimmune globulins from diverse B-cell repertoires.
Nature biotechnology
Keating SM,Mizrahi RA,Adams MS,Asensio MA,Benzie E,Carter KP,Chiang Y,Edgar RC,Gautam BK,Gras A,Leong J,Leong R,Lim YW,Manickam VA,Medina-Cucurella AV,Niedecken AR,Saini J,Simons JF,Spindler MJ,Stadtmiller K,Tinsley B,Wagner EK,Wayham N,Tracy L,Lundberg CV,Büscher D,Terencio JV,Roalfe L,Pearce E,Richardson H,Goldblatt D,Ramjag AT,Carrington CVF,Simmons G,Muench MO,Chamow SM,Monroe B,Olson C,Oguin TH,Lynch H,Jeanfreau R,Mosher RA,Walch MJ,Bartley CR,Ross CA,Meyer EH,Adler AS,Johnson DS
A-21281 was used in Sample Prep to describe a microfluidics and molecular genomics strategy for capturing diverse mammalian antibody repertoires to create recombinant multivalent hyperimmune globulins.
Sun Aug 01 00:00:00 EDT 2021
Functional diversity for body actions in the mesencephalic locomotor region.
Cell
Ferreira-Pinto MJ,Kanodia H,Falasconi A,Sigrist M,Esposito MS,Arber S
A-21281 was used in Immunohistochemistry to identify and functionally dissect glutamatergic mesencephalic locomotor region subpopulations based on the premise that target connectivity might be linked to function.
Thu Aug 19 00:00:00 EDT 2021
Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Balic Z,Misra S,Willard B,Reinhardt DP,Apte SS,Hubmacher D
A-21281 was used in Immunocytochemistry to identify a novel disulfide-bridged insertion in the ADAMTS17A spacer that originates from inclusion of a novel exon.
Mon Feb 01 00:00:00 EST 2021
An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategy.
Communications biology
Hunter SA,McIntosh BJ,Shi Y,Sperberg RAP,Funatogawa C,Labanieh L,Soon E,Wastyk HC,Mehta N,Carter C,Hunter T,Cochran JR
A-21281 was used in Flow cytometry/Cell sorting to investigate if ligand traps are a promising therapeutic strategy for cancer treatment.
Mon Apr 12 00:00:00 EDT 2021
Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms.
Nature communications
Shi X,Wan Y,Wang N,Xiang J,Wang T,Yang X,Wang J,Dong X,Dong L,Yan L,Li Y,Liu L,Hou S,Zhong Z,Wilson IA,Yang B,Yang G,Lerner RA
A-21281 was used in Flow cytometry/Cell sorting to describe an epitope-guided approach for selection of antibodies that modulate cellular signaling of targeted receptors.
Wed May 05 00:00:00 EDT 2021
Engineering a potent receptor superagonist or antagonist from a novel IL-6 family cytokine ligand.
Proceedings of the National Academy of Sciences of the United States of America
Kim JW,Marquez CP,Sperberg RAP,Wu J,Bae WG,Huang PS,Sweet-Cordero EA,Cochran JR
A-21281 was used in Immunocytochemistry to validate the CLCF1-CNTFR signaling axis as a therapeutic target and highlight an approach of engineering cytokine activity through a small number of mutations.
Tue Jun 23 00:00:00 EDT 2020
Genetically encoded live-cell sensor for tyrosinated microtubules.
The Journal of cell biology
Kesarwani S,Lama P,Chandra A,Reddy PP,Jijumon AS,Bodakuntla S,Rao BM,Janke C,Das R,Sirajuddin M
A-21281 was used in Flow cytometry/Cell sorting to describe a novel tyrosination sensor and its potential applications to study the dynamics of microtubules and their post-translational modification processes in living cells.
Mon Oct 05 00:00:00 EDT 2020
Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR.
mAbs
Simons JF,Lim YW,Carter KP,Wagner EK,Wayham N,Adler AS,Johnson DS
A-21281 was used in Flow cytometry/Cell sorting to find that improved affinity for the full-length antibody did not always translate into enhanced binding to cell-surface expressed antigen or improved immune checkpoint blocking ability, suggesting that screening with full-length antibody or antigen-binding fragment formats might be advantageous and the subject of a future study.
Mon Jul 05 00:00:00 EDT 2021
Small Molecule Dysregulation of TEAD Lipidation Induces a Dominant-Negative Inhibition of Hippo Pathway Signaling.
Cell reports
Holden JK,Crawford JJ,Noland CL,Schmidt S,Zbieg JR,Lacap JA,Zang R,Miller GM,Zhang Y,Beroza P,Reja R,Lee W,Tom JYK,Fong R,Steffek M,Clausen S,Hagenbeek TJ,Hu T,Zhou Z,Shen HC,Cunningham CN
A-21281 was used in Western Blot to demonstrate that targeting the TEAD LP is a promising therapeutic strategy for modulating the Hippo pathway, showing tumor stasis in a mouse xenograft model.
Tue Jun 23 00:00:00 EDT 2020
The Cellular Protein CAD is Recruited into Ebola Virus Inclusion Bodies by the Nucleoprotein NP to Facilitate Genome Replication and Transcription.
Cells
Brandt J,Wendt L,Bodmer BS,Mettenleiter TC,Hoenen T
A-21281 was used in Immunocytochemistry to analyze the functional and molecular interactions between Ebola virus and dihydroorotase.
Fri May 01 00:00:00 EDT 2020
Yeast surface display of full-length human microtubule-associated protein tau.
Biotechnology progress
Wang S,Cho YK
A-21281 was used in Immunocytochemistry to show that yeast-displayed tau enables robust measurement of protein interactions and is of particular interest for characterizing conformational change.
Wed Jan 01 00:00:00 EST 2020
An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA.
Scientific reports
Mehta N,Maddineni S,Kelly RL,Lee RB,Hunter SA,Silberstein JL,Parra Sperberg RA,Miller CL,Rabe A,Labanieh L,Cochran JR
A-21281 was used in Flow cytometry/Cell sorting to report the use of yeast surface display to engineer an anti-VISTA antibody that binds with high affinity to mouse, human, and cynomolgus monkey VISTA.
Wed Sep 16 00:00:00 EDT 2020
Antitumor activity of an engineered decoy receptor targeting CLCF1-CNTFR signaling in lung adenocarcinoma.
Nature medicine
Kim JW,Marquez CP,Kostyrko K,Koehne AL,Marini K,Simpson DR,Lee AG,Leung SG,Sayles LC,Shrager J,Ferrer I,Paz-Ares L,Gephart MH,Vicent S,Cochran JR,Sweet-Cordero EA
A-21281 was used in Flow cytometry/Cell sorting to nominate blockade of CLCF1-CNTFR signaling as a novel therapeutic opportunity for LUAD and potentially for other tumor types in which CLCF1 is present in the tumor microenvironment.
Fri Nov 01 00:00:00 EDT 2019
Antibody repertoire analysis of mouse immunization protocols using microfluidics and molecular genomics.
mAbs
Asensio MA,Lim YW,Wayham N,Stadtmiller K,Edgar RC,Leong J,Leong R,Mizrahi RA,Adams MS,Simons JF,Spindler MJ,Johnson DS,Adler AS
A-21281 was used in Flow cytometry/Cell sorting to conclude that modern microfluidic methods, followed by an extensive molecular genomic analysis of antibody repertoires, can be used to quickly analyze new immunization protocols or mouse platforms.
Mon Jul 01 00:00:00 EDT 2019
Directed Evolution of Split APEX2 Peroxidase.
ACS chemical biology
Han Y,Branon TC,Martell JD,Boassa D,Shechner D,Ellisman MH,Ting A
A-21281 was used in Flow cytometry/Cell sorting to demonstrate sAPEX reconstitution in the mammalian cytosol, on engineered RNA motifs within a non-coding RNA scaffold, and at mitochondria-endoplasmic reticulum contact sites.
Fri Apr 19 00:00:00 EDT 2019
Engineering of a novel subnanomolar affinity fibronectin III domain binder targeting human programmed death-ligand 1.
Protein engineering, design & selection : PEDS
Ramakrishnan S,Natarajan A,Chan CT,Panesar PS,Gambhir SS
A-21281 was used in Flow cytometry/Cell sorting to engineer a subnanomolar, high-affinity fibronectin type 3 domain (FN3)-based small binder targeted against human PD-L1 (hPD-L1) present on tumor cells.
Tue Dec 31 00:00:00 EST 2019
Evaluation of integrin αvβ6 cystine knot PET tracers to detect cancer and idiopathic pulmonary fibrosis.
Nature communications
Kimura RH,Wang L,Shen B,Huo L,Tummers W,Filipp FV,Guo HH,Haywood T,Abou-Elkacem L,Baratto L,Habte F,Devulapally R,Witney TH,Cheng Y,Tikole S,Chakraborti S,Nix J,Bonagura CA,Hatami N,Mooney JJ,Desai T,Turner S,Gaster RS,Otte A,Visser BC,Poultsides GA,Norton J,Park W,Stolowitz M,Lau K,Yang E,Natarajan A,Ilovich O,Srinivas S,Srinivasan A,Paulmurugan R,Willmann J,Chin FT,Cheng Z,Iagaru A,Li F,Gambhir SS
A-21281 was used in Flow cytometry/Cell sorting to describe the engineering and validation of a new cystine knot peptide that selectively recognises human integrin αvβ6 with single-digit nanomolar affinity.
Mon Oct 14 00:00:00 EDT 2019
A natively paired antibody library yields drug leads with higher sensitivity and specificity than a randomly paired antibody library.
mAbs
Adler AS,Bedinger D,Adams MS,Asensio MA,Edgar RC,Leong R,Leong J,Mizrahi RA,Spindler MJ,Bandi SR,Huang H,Tawde P,Brams P,Johnson DS
A-21281 was used in Flow cytometry/Cell sorting to demonstrate that natively paired libraries have critical advantages in sensitivity and specificity for antibody discovery programs.
Sun Apr 01 00:00:00 EDT 2018
Adnectin-drug conjugates for Glypican-3-specific delivery of a cytotoxic payload to tumors.
Protein engineering, design & selection : PEDS
Lipovšek D,Carvajal I,Allentoff AJ,Barros A,Brailsford J,Cong Q,Cotter P,Gangwar S,Hollander C,Lafont V,Lau WL,Li W,Moreta M,O'Neil S,Pinckney J,Smith MJ,Su J,Terragni C,Wallace MA,Wang L,Wright M,Marsh HN,Bryson JW
A-21281 was used in Flow cytometry/Cell sorting to develop Adnectin-drug conjugates as an alternative platform for tumour-specific delivery of cytotoxic payloads.
Tue May 01 00:00:00 EDT 2018
Targeting the Tie2-αvβ3 integrin axis with bi-specific reagents for the inhibition of angiogenesis.
BMC biology
Shlamkovich T,Aharon L,Koslawsky D,Einav Y,Papo N
A-21281 was used in Flow cytometry/Cell sorting to target the Tie2-αvΒ3 integrin axis with bi-specific reagents for the inhibition of angiogenesis.
Fri Aug 17 00:00:00 EDT 2018
EMC3 coordinates surfactant protein and lipid homeostasis required for respiration.
The Journal of clinical investigation
Tang X,Snowball JM,Xu Y,Na CL,Weaver TE,Clair G,Kyle JE,Zink EM,Ansong C,Wei W,Huang M,Lin X,Whitsett JA
A-21281 was used in Immunohistochemistry to demonstrate that EMC3 is required for murine pulmonary surfactant synthesis and lung function at birth.
Fri Dec 01 00:00:00 EST 2017
Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis.
Frontiers in immunology
Tiller KE,Chowdhury R,Li T,Ludwig SD,Sen S,Maranas CD,Tessier PM
A-21281 was used in Flow cytometry/Cell sorting to develop an efficient methodology for systematically and rapidly enhancing the affinity of antibody variable domains while maximising specificity and stability using novel synthetic antibody libraries.
Wed Nov 20 00:00:00 EST 2019
Dual display of proteins on the yeast cell surface simplifies quantification of binding interactions and enzymatic bioconjugation reactions.
Biotechnology journal
Lim S,Glasgow JE,Filsinger Interrante M,Storm EM,Cochran JR
A-21281 was used in Flow cytometry/Cell sorting to develop a novel yeast display strategy that comprises fusion of two different proteins to Aga2p.
Mon May 01 00:00:00 EDT 2017
Delineating antibody recognition against Zika virus during natural infection.
JCI insight
Yu L,Wang R,Gao F,Li M,Liu J,Wang J,Hong W,Zhao L,Wen Y,Yin C,Wang H,Zhang Q,Li Y,Zhou P,Zhang R,Liu Y,Tang X,Guan Y,Qin CF,Chen L,Shi X,Jin X,Cheng G,Zhang F,Zhang L
A-21281 was used in Flow cytometry/Cell sorting to understand the pathogenesis of Zika virus infection and inform rational design of vaccines.
Thu Jun 15 00:00:00 EDT 2017
Miro phosphorylation sites regulate Parkin recruitment and mitochondrial motility.
Proceedings of the National Academy of Sciences of the United States of America
Shlevkov E,Kramer T,Schapansky J,LaVoie MJ,Schwarz TL
A21281 was used in immunocytochemistry to study the role of Miro phosphorylation in Parkin-dependent mitochondrial arrest
Tue Oct 11 00:00:00 EDT 2016
Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals.
mAbs
Wang B,Lee CH,Johnson EL,Kluwe CA,Cunningham JC,Tanno H,Crooks RM,Georgiou G,Ellington AD
A-21281 was used in flow cytometry to assess the discovery of high affinity anti-ricin antibodies by yeast display of combinatorial VH:VL libraries from immunized animals and B cell receptor sequencing
Wed Dec 06 00:00:00 EST 2017
SIRT2 inactivation reveals a subset of hyperacetylated perinuclear microtubules inaccessible to HDAC6.
Journal of cell science
Skoge RH,Ziegler M
A-21281 was used in Immunocytochemistry to examine whether NAD-dependent deacetylase (SIRT2) and the NAD-independent deacetylase (HDAC6) have distinguishable preferences for the deacetylation of different microtubule structures.
Mon Aug 01 00:00:00 EDT 2016
Conformation-dependent epitopes recognized by prion protein antibodies probed using mutational scanning and deep sequencing.
Journal of molecular biology
Doolan KM,Colby DW
A-21281 was used in Flow cytometry/Cell sorting to screen 1341 prion protein single point mutants for attenuated interaction with four anti-PrP antibodies, including several with conformational specificity.
Fri Jan 30 00:00:00 EST 2015
Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities.
Methods in molecular biology (Clifton, N.J.)
Zhang K,Bhuripanyo K,Wang Y,Yin J
A-21281 was used in flow cytometry to discuss the key steps of enzyme engineering on the yeast cell surface.
Tue Mar 15 00:00:00 EDT 2016
Changing the peptide specificity of a human T-cell receptor by directed evolution.
Nature communications
Smith SN,Wang Y,Baylon JL,Singh NK,Baker BM,Tajkhorshid E,Kranz DM
A-21281 was used in Immunocytochemistry-immunoflourescence to develop a strategy for using in vitro-directed evolution to alter the peptide specificity of a human T-cell receptor.
Fri Nov 07 00:00:00 EST 2014
Protein selection using yeast surface display.
Methods (San Diego, Calif.)
Gera N,Hussain M,Rao BM
A-21281 was used in flow cytometry to discuss the use of yeast surface display to isolate binding proteins from combinatorial libraries.
Fri Mar 15 00:00:00 EDT 2013
MAZe: a tool for mosaic analysis of gene function in zebrafish.
Nature methods
Collins RT,Linker C,Lewis J
A-21281 was used in immunohistochemistry to create tools to help make inducible knockout zebrafish
Mon Mar 01 00:00:00 EST 2010
Biosensor detection systems: engineering stable, high-affinity bioreceptors by yeast surface display.
Methods in molecular biology (Clifton, N.J.)
Richman SA,Kranz DM,Stone JD
A-21281 was used in flow cytometry to use yeast surface display techniques improve affinity and stability of bioreceptors
Thu Mar 19 00:00:00 EDT 2009
Construction and screening of antigen targeted immune yeast surface display antibody libraries.
Current protocols in cytometry
Miller KD,Pefaur NB,Baird CL
A-21281 was used in flow cytometry to describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice
Tue Jul 01 00:00:00 EDT 2008
Isolating and engineering human antibodies using yeast surface display.
Nature protocols
Chao G,Lau WL,Hackel BJ,Sazinsky SL,Lippow SM,Wittrup KD
A-21281 was used in flow cytometry to use yeast surface display to isolate and engineer antibodies or proteins for increased affinity and stability
Fri Sep 21 00:00:00 EDT 2007
Poly(beta-amino ester)s promote cellular uptake of heparin and cancer cell death.
Chemistry & biology
Berry D,Lynn DM,Sasisekharan R,Langer R
A-21281 was used in immunohistochemistry to investigate the consequences of heparin and heparan sulfate-like glycosaminoglycan internalization
Thu Apr 01 00:00:00 EST 2004
Nuclear localization of a non-caspase truncation product of atrophin-1, with an expanded polyglutamine repeat, increases cellular toxicity.
The Journal of biological chemistry
Nucifora FC,Ellerby LM,Wellington CL,Wood JD,Herring WJ,Sawa A,Hayden MR,Dawson VL,Dawson TM,Ross CA
A-21281 was used in immunocytochemistry to assess the increase in cellular toxicity by nuclear localization of a non-caspase truncation product of atrophin-1 with an expanded polyglutamine repeat
Fri Apr 11 00:00:00 EDT 2003
